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Author:
Date:
2015-07-20
[Abstract] Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as heavy metal or oxidative stress. The protocol was extensively used to phenotype a collection of gene deletion mutants in the human fungal pathogen Candida glabrata (C. glabrata) (Schwarzmüller et al., 2014).
[摘要] 在这里，我们描述了一种使表型分析的基因组规模删除集合的真菌突变体检测表型的各种应力条件的方法。 这些胁迫条件包括许多其它抗真菌药物敏感性，温度诱导和渗透以及重金属或氧化应激。 该方案广泛用于表达人真菌病原体光滑假丝酵母（ glabrata ）中基因缺失突变体的集合（Schwarzmüller等人 >，2014）。
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