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Author:
Date:
2015-07-05
[Abstract] Cassava (Manihot esculenta) is a root crop that provides calories for people living in more than 100 tropical and subtropical countries and serves as a raw material for processing into starch and biofuels as well as feed for livestock (Howeler et al., 2013). Xanthomonas axonopodis pv. manihotis (Xam), the causal agent of cassava bacterial blight (CBB), can cause extensive crop damage (reviewed in Lopez et al., 2012; Lozano, 1986). Bacterial movement, growth in planta and the ability to cause disease symptoms are all important measures of bacterial fitness and plant susceptibility to CBB. Here we present a protocol for visualizing the movement of Xam within the plant. We also provide a detailed method of assaying bacterial growth in the ...
[摘要] 木薯（ Manihot esculenta ）是一种根茎作物，为生活在100多个热带和亚热带国家的人们提供热量，并且作为加工成淀粉和生物燃料以及牲畜饲料的原料（Howeler et al。，2013）。 Xanthomonas axonopodis pv。木薯细菌枯萎病（CBB）的致病因子可引起广泛的作物损害（参见Lopez等人，2012; Lozano，1986）。细菌运动，植物中的生长和引起疾病症状的能力都是细菌适合性和植物对CBB敏感性的重要测量。在这里，我们提出了一个用于可视化植物中的Xam 运动的协议。我们还提供了一种详细的方法来检测木薯叶中期的细菌生长，以及叶片外质层中的细菌生长和疾病症状发展。这些方法将是确定Xam 菌株致病性和开发对CBB有抗性的木薯品种的重要工具。
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