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Salmon Sperm DNA, sheared (10 mg/mL)

鲑鱼精子DNA,剪切(10mg / mL)

Company: Thermo Fisher Scientific
Catalog#: AM9680
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Quantifying Symmetrically Methylated H4R3 on the Kaposi’s Sarcoma-associated Herpesvirus (KSHV) Genome by ChIP-Seq
Author:
Date:
2018-03-20
[Abstract]  Post-translational modifications to histone tails contribute to the three-dimensional structure of chromatin and play an important role in determining the relative expression of nearby genes. One such modification is symmetric di-methylation of arginine residues, which may exhibit different effects on gene expression including blocking the binding of transcriptional activators, or recruiting repressive effector molecules. Recent ChIP-Seq studies have demonstrated the importance of cross-talk between different histone modifications in gene regulation. Thus, to acquire a comprehensive understanding of the combined efforts of these epigenetic marks, ChIP-Seq must be utilized for identifying specific enrichment on the chromatin. Tumorigenic herpesvirus KSHV, employs epigenetic mechanisms for ... [摘要]  组蛋白尾部的翻译后修饰有助于染色质的三维结构,并在确定附近基因的相对表达中起重要作用。一种这样的修饰是精氨酸残基的对称二甲基化,其可能对基因表达展现出不同的作用,包括阻断转录激活剂的结合或招募抑制效应分子。最近的ChIP-Seq研究已经证明不同组蛋白修饰之间在基因调节中的相互作用的重要性。因此,为了全面了解这些表观遗传标记的共同努力,必须利用ChIP-Seq来鉴定染色质上的特定富集。利用表观遗传机制进行基因调控,并且通过ChIP-Seq以全面,不偏倚的方式评估多种组蛋白修饰的相对丰度,我们可以获得关于病毒复制和发病机理的复杂机制的更好的见解。

【背景】卡波西肉瘤相关疱疹病毒(KSHV)是一种致癌性人类病毒,在其生命周期中有两个不同阶段。在最初感染后,KSHV在宿主中建立持续的终生感染,这对免疫受损的个体来说特别有问题。 KSHV可引起HIV / AIDS患者中的各种肿瘤,包括卡波西肉瘤和多发性B细胞淋巴瘤(Chang等,1994; Cesarman等,1995; Russo ,1996; Soulier ,1995)。 KSHV拥有大约165,000bp的大型基因组,编码近90种不同的开放阅读框,具有逃避宿主免疫监视系统,改变宿主细胞生长途径和产生感染性后代病毒粒子的充足工具。

在潜伏阶段期间,仅有一部分病毒基因被表达,其在本质上是致癌的并且还有助于病毒附加体复制和传递到分裂的肿瘤细胞中(Uppal等人,2014; ...

Staining of Membrane Receptors with Fluorescently-labeled DNA Aptamers for Super-resolution Imaging
Author:
Date:
2017-09-05
[Abstract]  One of the most prominent applications of fluorescent super-resolution microscopy is the study of nanodomain arrangements of receptors and the endocytic pathway. Staining methods are becoming crucial for answering questions on the nanoscale, therefore, the use of small and monovalent affinity probes is of great interest in super-resolution microscopy with biological samples. One kind of affinity probe is the aptamer. Aptamers are single DNA or RNA sequences that bind with high affinity to their targets and due to their small size they are able to (i) place the fluorophore in close proximity to the protein of interest and (ii) bind to most of the protein of interest overcoming the steric hindrance effect, resulting in better staining density. Here we describe a detailed protocol with which ... [摘要]  荧光超分辨率显微镜的最突出的应用之一是研究纳米结构的受体和内吞途径。染色方法对于回答纳米尺度的问题变得至关重要,因此,使用小型和单价亲和探针在具有生物样品的超分辨显微镜中是非常有意义的。一种亲和力探针是适体。适配体是以高亲和力结合其靶标的单个DNA或RNA序列,并且由于它们的小尺寸,它们能够(i)将荧光团置于感兴趣的蛋白质附近,并且(ii)与大部分蛋白质结合有利于克服空间位阻效应,导致更好的染色密度。在这里,我们描述一个详细的协议,使用适配体染色活细胞,并用刺激的排放消耗(STED)显微镜对其进行成像。在该方案中,染色用市售适用于靶向表皮生长因子受体(EGFR),人表皮生长因子受体2(HER2或ErbB2)和ephrin-A受体2(Epha2)的适体进行。由于适配体可与大部分受欢迎的荧光团偶联,因此我们认为本文介绍的方法可扩展到目前超分辨率显微镜技术的绝大多数。
【背景】超分辨率成像技术的最新进展已经导致搜索更精确的方法来标记细胞元件。衍射无限成像仪器提供优异的分辨率,然而标准样品染色方法,如免疫染色,缺乏检测细胞元素所必需的精度。由于它们的大尺寸(长度约15nm)和高分子量(〜150kDa),抗体可以很差地渗透到生物样品中。另外,一次/二次抗体复合物将荧光团放置在远离靶的大约25nm处,从而损害检测精度。此外,由于初级/二级抗体复合物的大尺寸,由于空间位阻(Fornasiero和Opazo,2015),较小部分的靶标可以被标记。这导致较低的标记密度,这是超分辨率显微镜的关键参数,特别是在识别和描述纳米结构时。为了克服这些问题,近年来已经测试了与单个靶(单价)结合的小的亲和力探针,如适体,亲和体或纳米体系(Rothbauer ...

In vitro Drug Susceptibility Assay for HBV Using Southern Blotting
Author:
Date:
2015-04-20
[Abstract]  Antiviral agents for the suppression of hepatitis B virus (HBV) have been used for treating chronic hepatitis B. However, the emergence of drug-resistant HBV is still a major problem for antiviral treatment. To identify and characterize the drug-resistant HBV, the construction of HBV replicon and in vitro drug susceptibility assay are essential. Here we describe the experimental methods to study drug-resistant HBV. [摘要]  用于抑制乙型肝炎病毒(HBV)的抗病毒剂已经用于治疗慢性乙型肝炎。然而,耐药性HBV的出现仍然是抗病毒治疗的主要问题。 为了鉴定和表征耐药性HBV,构建HBV复制子和体外药物敏感性测定是必需的。 在这里我们描述研究耐药性HBV的实验方法。

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