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Author:
Date:
2015-06-20
[Abstract] To study the functions of sugars in plants, feeding experiment is one of the most common and easy methods. However, the traditional method, e.g., a floating of leaf discs on sugar-containing solution seems to have an insufficient efficiency of sugar uptake, despite of high osmotic and injury effects. This is a protocol to feed oligosaccharide gentiobiose into in vitro cultured tissues of gentian. This protocol enables to incorporate gentiobiose into intact tissues without exposure to osmotic stress and may be useful to other plant species that are able to propagate by shoot tip culture.
[摘要] 为了研究植物中糖的功能，饲养实验是最常见和最容易的方法之一。 然而，传统方法，例如，在含糖溶液上漂浮叶盘似乎具有不充分的糖吸收效率，尽管具有高的渗透和损伤效应。 这是一种将寡糖龙胆二糖供给到体外培养的龙胆的组织中的方案。 该方案能够将龙胆二糖掺入完整组织而不暴露于渗透胁迫，并且可以用于能够通过芽尖培养繁殖的其它植物物种。
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