Preparation and Characterization of Ginger Lipid-derived Nanoparticles for Colon-targeted siRNA Delivery
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Author:
Date:
2020-07-20
[Abstract] Synthetic nanoparticle-based drug delivery system is widely known for its ability to increase the efficacy and specificity of loaded drugs, but it often suffers from relatively higher immunotoxicity and higher costs as compared to traditional drug formulations. Contrarily, plant-derived nanoparticles appear to be free from these limitations of synthetic nanoparticles; they are naturally occurring biocompatible vesicles that do not generate immunotoxicity and are easy to obtain. Additionally, lipids isolated from plant-derived nanoparticles have shown the capability of assembling themselves to spherical nano-sized liposomal particles. Herein, we employ lipids extracted from ginger-derived nanoparticles and load them with therapeutic siRNA (CD98-siRNA) to create CD98-siRNA/ginger-lipid ...
[摘要] [摘要 ]基于合成纳米颗粒的药物递送系统以其增加所载药物的功效和特异性的能力而广为人知,但是与传统药物制剂相比,它经常遭受相对较高的免疫毒性和较高的成本。相反,植物来源的纳米粒子似乎不受合成纳米粒子的这些限制;它们是天然存在的生物相容性囊泡,不会产生免疫毒性并且易于获得。另外,从植物来源的纳米颗粒分离的脂质已显示出将其自身组装成球形纳米尺寸脂质体颗粒的能力。在本文中,我们采用从生姜纳米颗粒中提取的脂质,并用治疗性siRNA(CD98-siRNA)加载脂质,以创建CD98-siRNA /生姜脂质纳米颗粒。CD98-siRNA /姜脂脂质纳米颗粒的表征显示它们呈球形,直径约为189.5 nm。纳米颗粒的表面ζ电势在-18.1至-18.4mV之间变化。此外,在最近的研究中,CD98-siRNA /姜脂纳米颗粒在右旋糖酐硫酸钠(DSS)诱发的结肠炎小鼠模型中显示出特定的结肠靶向能力和出色的抗炎功效。
[背景 ] 小干扰RNA(siRN As)是一种有前途的治疗剂,可以通过沉默异常上调的信使RNA(mRNA)来治疗各种疾病(Nikam and Gore ,2018)。尽管siRNA的有效性,安全有效地将siRNA递送至治疗靶标仍然是一项艰巨的任务(Tatiparti et al ...
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Growth Recovery Assay and FACS-based Population Sorting Following Territorial Exclusion in Proteus mirabilis
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Author:
Date:
2020-03-05
[Abstract] Many bacteria take part in self recognition and kin discrimination behavior using contact-dependent effectors. Understanding the effects these effectors cause is important to explain bacterial community formation and population dynamics. Typically, kin discrimination effectors are toxins that kill target cells; their effect is therefore obvious and easily measurable. However, many self-recognition effectors, such as the Proteus mirabilis Ids system, are non-lethal and do not cause obvious physiological changes in target cells. Previously, experimental techniques to probe cells experiencing non-lethal kin recognition have been limited. Here we describe a technique to reliably isolate cells deemed self and non-self through Ids self-recognition for downstream phenotypic analysis. ...
[摘要] [摘要] 许多细菌使用接触依赖性效应子参与自我识别和亲属歧视行为。了解这些效应子引起的作用对于解释细菌群落形成和种群动态很重要。通常,亲属歧视效应子是杀死靶细胞的毒素;因此,它们的效果是显而易见的,并且易于测量。但是,许多自我识别效应器,例如变形杆菌(Proteus mirabilis) Ids系统是非致命性的,不会在靶细胞中引起明显的生理变化。以前,探测经历非致命亲属识别的细胞的实验技术受到限制。在这里,我们描述了一种技术,该技术可通过Ids自我识别可靠地分离被视为自身和非自身的细胞,以进行下游表型分析。将荧光标记的自我识别突变体的液体培养物混合在一起,并接种在群体允许的琼脂上。收获混合群,并通过荧光激活细胞分选术(FACS)分离每个菌株。在平板读取器上测量每种菌株的生长速率。该协议适用于其他细菌物种。我们简要描述了如何将分类的颗粒用于其他分析,如RNA-Seq文库制备。
[背景和 d] 许多生物的进化适应社区生活。整个大自然中存在的一种常见机制是亲属歧视:对近亲的优先对待和对非亲属的阻碍(Smith,1964)。细菌亲属歧视的研究充分的例子包括接触依赖性抑制(CDI)(Aoki 等,2009; Garcia 等,2016)和通过IV型和VI型分泌系统进行毒素转移(Brunet 等,2013; Souza ...
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Quantification of Ralstonia solanacearum Colonization of Potato Germplasm Using Luminescence
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Author:
Date:
2015-05-05
[Abstract] We have developed an unsophisticated, non-disruptive and accurate method for evaluation of pathogen colonization in planta. In this protocol we use a Ralstonia solanacearum (R. solanacearum) UY031 strain genetically modified to constitutively generate light from a synthetic luxCDABE operon stably inserted in its chromosome. This system allows bacterial quantification in a high-throughput manner, avoiding time-consuming and tedious bacterial dilution plating and colony counting. In addition, this system could be especially useful in plant breeding programs to detect bacterial latent growth in symptomless parental lines before their inclusion in long-term disease resistance breeding programs.
[摘要] 我们已经开发了一种不成熟的,非破坏性和准确的方法用于评估植物中的病原体定居。 在该方案中,我们使用经基因修饰以从稳定插入的合成的luxCDABE 操纵子组成性地产生光的(R)solanacearum 其染色体。 该系统允许以高通量方式进行细菌定量,避免耗时和繁琐的细菌稀释电镀和菌落计数。 此外,该系统可以在植物育种程序中特别有用,以在其包括在长期疾病抗性育种程序中之前检测无症状亲本细胞中的细菌潜伏生长。
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