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Author:
Date:
2015-04-05
[Abstract] Karyogamy, a migration of the sperm nucleus toward the egg nucleus and their subsequent nuclear fusion, is an important biological event for initiating zygote formation toward early embryogenesis in angiosperms. However, how the male nucleus approaches and fuses with the female nucleus still remains unclear. Recently, time-lapse measurement of nuclear volume during karyogamic events revealed that the sperm nucleus enlarges during contact with the egg nucleus via possible one-directional migration of egg chromatin into sperm nucleus (Ohnishi et al., 2014). Here, we describe the protocol for microscopic observation, three-dimensional reconstruction, and volume measurements of sperm nuclei in rice zygotes/fused gametes, which are produced by an in vitro fertilization system ...
[摘要] 核型，精子核朝向卵核和它们随后的核融合的迁移是引起被子植物中早期胚胎形成的合子形成的重要生物事件。然而，男性核如何接近和融合与女性核仍然不清楚。最近，核染色体事件中核体积的延时测量显示精子核在与卵核接触期间扩大，通过卵染色质可能的一向迁移到精子核中（Ohnishi等人，2014年， ）。在这里，我们描述了用于显微观察，三维重建和体积测量的水稻合子/融合配子中的精子细胞核的方案，其由体外受精系统产生（Uchiumi等人al。，2006; Uchiumi等人，2007）。本协议将适用于监测细胞分裂，分化，去分化和极性形成以及核型进展期间细胞中的核动力学。
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