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Author:
Date:
2015-04-05
[Abstract] Bacterial glycoproteins are of increasing interest due to their abundance in nature and importance in health and infectious diseases. However, only a very small fraction of bacterial glycoproteins have been characterized and its post-translational modification machinery identified. While analysis of glycoproteins can be achieved through various techniques, this is often limited by the specific characteristics of individual proteins such as type and level of glycosylation. Lectins are sugar-binding proteins that recognize specific glycoconjugates in a manner similar to antigen-antibody interactions. Here, we describe a simple method for the detection of glycoproteins using lectin-based Western blot analysis, which can be applied to different organisms and coupled with various other ...
[摘要] 细菌糖蛋白由于其在自然界中的丰富性和在健康和传染病中的重要性而日益受到关注。 然而，只有非常小部分的细菌糖蛋白已被表征和其翻译后修饰机制鉴定。 尽管通过各种技术可以实现糖蛋白的分析，但是这常常受到单个蛋白质的特定特征的限制，例如糖基化的类型和水平。 凝集素是以类似于抗原 - 抗体相互作用的方式识别特异性糖缀合物的糖结合蛋白。 在这里，我们描述了一种简单的方法使用基于凝集素的蛋白质印迹分析检测糖蛋白，这可以应用于不同的生物体，并与各种其他策略的补充分析。
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