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M-MLV Reverse Transcriptase

M-MLV逆转录酶

Company: Promega
Catalog#: 28025-013
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RNA Editing Detection by Direct Sequencing
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Date:
2015-03-05
[Abstract]  RNA editing is a widespread post-transcriptional phenomenon through which primary RNA sequences are altered by nucleotide insertion/deletion or base conversion. It occurs in a variety of organisms and cooperates with alternative splicing in increasing both proteomic and transcriptomic complexity. We describe here a method allowing RNA editing events detection by performing direct sequencing of both genomic DNA and cDNA from the same source. [摘要]  RNA编辑是广泛的转录后现象,通过其通过核苷酸插入/缺失或碱基转化来改变初级RNA序列。 它发生在多种生物体中,并与增加蛋白质组和转录组复杂性的可变剪接协作。 我们在这里描述一种允许通过对来自相同来源的基因组DNA和cDNA进行直接测序来进行RNA编辑事件检测的方法。

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