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Author:
Date:
2014-11-20
[Abstract] The polyamines, spermidine (Spd) and spermine, and their diamine precursor putrescine, are important regulators of various cellular functions, such as proliferation and differentiation. Polyamine homeostasis is tightly regulated on the level of uptake, excretion, biosynthesis, interconversion and terminal catabolism. The rate-controlling enzymes of polyamine biosynthesis and interconversion are ornithine decarboxylase (ODC) and spermidine/spermine N1-acetyltransferase (SSAT), respectively. Here, we describe a protocol to assay ODC (Jänne and Williams-Ashman, 1971) and SSAT (Libby, 1978) activities from cell or tissue samples.
[摘要] 多胺，亚精胺（精）和精胺及其二胺前体腐胺是各种细胞功能（如增殖和分化）的重要调节剂。 多胺稳态在摄取，排泄，生物合成，相互转化和末端分解代谢的水平上受到严格调节。 多胺生物合成和相互转化的速率控制酶分别是鸟氨酸脱羧酶（ODC）和亚精胺/精胺N 1 - 乙酰基转移酶（SSAT）。 在这里，我们描述了测定细胞或组织样品的ODC（Jänne和Williams-Ashman，1971）和SSAT（Libby，1978）活性的方案。
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