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Author:
Date:
2012-09-20
[Abstract] In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous methods (Thordal-Christensen et al., 1997; Bindschedler et al., 2006; Daudi et al., 2012). DAB is oxidized by hydrogen peroxide in the presence of some haem-containing proteins, such as peroxidases, to generate a dark brown precipitate. This precipitate is exploited as a stain to detect the presence and distribution of hydrogen peroxide in plant cells. The protocol can be modified slightly to detect hydrogen peroxide in different types of plant tissue.
[摘要] 在该方案中，在成熟的拟南芥花瓣叶中通过用3,3'-二氨基联苯胺（1mM）染色来描述过氧化氢（几种活性氧种类之一）的原位检测。 DAB），使用先前方法的适应（Thordal-Christensen等人，1997; Bindschedler等人，2006; Daudi等人， ，2012）。 DAB在一些含血红蛋白（例如过氧化物酶）的存在下被过氧化氢氧化，以产生深棕色沉淀。 利用该沉淀作为污渍来检测植物细胞中过氧化氢的存在和分布。 该方案可以稍微修改以检测不同类型的植物组织中的过氧化氢。
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