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Slab Gel dryer SGD5040

板式凝胶干燥机SGD5040

Company: Thermo Fisher Scientific
Catalog#: SGD5040-230
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In organello Protein Synthesis
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2014-06-20
[Abstract]  In organello protein synthesis method allows the analysis of mitochondrial translation products. The principle of this method relies on incubation of isolated intact mitochondria with radiolabeled amino acids such as 35S methionine. After protein synthesis, the radiolabeled translation products are subsequently separated by SDS polyacrylamide gel electrophoresis and analysed by autoradiography. For in organello analysis of protein synthesis, the isolated intact mitochondria must retain their bioenergetics capacity, and in consequence be fully functional and able to perform coupled respiration. This in turn requires a quick and gentle purification of mitochondria during their isolation. [摘要]  在organello中,蛋白质合成方法允许分析线粒体翻译产物。 该方法的原理依赖于分离的完整线粒体与放射性标记的氨基酸如35 S-甲硫氨酸的孵育。 在蛋白质合成之后,随后通过SDS聚丙烯酰胺凝胶电泳分离放射性标记的翻译产物,并通过放射自显影进行分析。 对于蛋白质合成的分析,分离的完整线粒体必须保持其生物能量能力,因此是完全功能的并且能够进行耦合呼吸。 这反过来需要在其分离期间快速和温和地纯化线粒体。

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