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Fluorescence microscope

显微镜

Company: Olympus
Catalog#: DP71
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Direct Visualization of the Multicopy Chromosomes in Cyanobacterium Synechococcus elongatus PCC 7942
Author:
Date:
2018-08-05
[Abstract]  Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942 also contains multiple chromosomal copies per cell at all stages of its cell cycle. Here, we describe a method for the direct visualization of multicopy chromosomes in S. elongatus 7942 by fluorescence in situ hybridization (FISH). [摘要]  蓝细菌是进行含氧光合作用的原核生物。 淡水蓝藻 Synechococcus elongatus PCC 7942是用于研究光合作用和基因调控以及生物技术应用的模式生物。 除了几种淡水蓝藻, S. 细胞 7942在其细胞周期的所有阶段每个细胞还含有多个染色体拷贝。 在这里,我们描述了一种直接可视化 S中多拷贝染色体的方法。 通过荧光原位杂交(FISH)测定细菌 7942。

【背景】蓝藻是原核微生物,其利用与高等植物中的叶绿体类似的产氧光合系统。虽然 Escherichia coli 和枯草芽孢杆菌等细菌具有单个圆形染色体,但是几种淡水蓝细菌每个细胞有多个圆形染色体(Mann和Carr,1974; Labarre 等人,1989; Binder和Chisholm,1995)。淡水蓝藻 Synechococcus elongatus PCC 7942(以下简称 S.longatus 7942)每个细胞携带3-8个染色体拷贝(Griese et al。,2011; Watanabe et al。,2012; Watanabe et al。,2015)。已经建立了荧光报告子 - 操纵子系统来获取 S的多拷贝染色体的图像。 elongatus 7942(Chen et al。,2012; Jain et ...

Visualising Differential Growth of Arabidopsis Epidermal Pavement Cells Using Thin Plate Spline Analysis
Author:
Date:
2016-11-20
[Abstract]  Epidermal pavement cells in Arabidopsis leaves and cotyledons develop from relatively simple shapes to form complex cells that have multiple undulations of varying sizes. Analyzing the growth of individual parts of the cell wall boundaries over time is essential to understanding how pavement cells develop their complex shapes. Thin plate spline analysis is a method for visualizing the change of size and shape of objects through warping or deformation of a regular mesh and can be applied to understand cell wall growth. This protocol describes the application of thin plate spline analysis to visualize the development of individual pavement cells over time. [摘要]  拟南芥叶和子叶中的表皮铺路细胞从相对简单的形状发育而形成具有不同大小的多个起伏的复合细胞。分析细胞壁边界的各个部分随时间的生长对理解铺路细胞如何发展其复杂形状是至关重要的。薄板样条分析是通过规则网格的翘曲或变形来可视化物体的尺寸和形状的变化的方法,并且可以用于理解细胞壁生长。该协议描述了薄板样条分析的应用,以便随时间可视化单个路面细胞的发育。

[背景] 了解细胞生长的空间模式提供了洞察植物细胞如何形成不同的形状。拟南芥子叶和叶的表皮铺路细胞是用于研究复杂细胞如何生长的良好模型系统,因为它们的细胞壁边界从最初为简单弧的边界开始形成不同大小的多个起伏(Armor et al。,2015; Fu et al。,2005)。通过将外部施加的标记物固定到细胞例如藻类氮细胞节间(Green等人,1970),根细胞(Shaw等人, ,2000)和毛状体(Schwab等人,2003)。然而,从外部施加的界标测量细胞生长有时是不可行的,例如当外部施加的荧光标记物的强荧光会遮蔽细胞内荧光标记的细胞骨架元件时(Armor等人,2015)。显示定义数量的同源界标随时间或在不同物体之间的变化位置的薄板样条分析先前已用于分析诸如人类头骨的物体的三维大小和形状的变化(Rosas和Bastir,2002; Gunz ,2014)和叶子(Polder et al ...

Karyotype Analysis
Author:
Date:
2014-05-20
[Abstract]  A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to be one of the hallmarks of tumor formation. Here we use the karyotype analysis to separate the chromosomes and observe the chromosomes in tumor cells with a microscope.
[摘要]  染色体是组织细胞中的DNA和蛋白质的结构。 它是含有编码和非编码序列的单片卷曲DNA。 人类细胞具有23对染色体,包括22对常染色体和一对性染色体,每个细胞总共46个染色体。 在肿瘤细胞中,染色体不稳定性已被认为是肿瘤形成的标志之一。 这里我们使用核型分析来分离染色体,并用显微镜观察肿瘤细胞中的染色体

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