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Author:
Date:
2014-05-05
[Abstract] Vaccine-based immunotherapy is being used to treat dogs with primary brain tumors. The vaccines are composed of a lysate of autologous tumor cells, which stimulate an immune response producing tumor specific antibodies that are capable of inducing antibody-dependent cell-mediated cytotoxicity to allogeneic, as well as autologous, tumor cells. This protocol will describe the tumor cell serum antibody-binding assay to measure the tumor-reactive IgG antibody response. Key features of this assay are that it is performed with sera collected from the canine patient prior to and following vaccination as the source of antibodies and canine brain tumor cells used as the target cells.
[摘要] 基于疫苗的免疫疗法被用于治疗具有原发性脑肿瘤的狗。 疫苗由自体肿瘤细胞的裂解物组成，其刺激产生免疫应答的肿瘤特异性抗体，其能够诱导对同种异体以及自体的肿瘤细胞的抗体依赖性细胞介导的细胞毒性。 该方案将描述肿瘤细胞血清抗体结合测定以测量肿瘤反应性IgG抗体应答。 该测定的主要特征是，在接种疫苗之前和之后从犬患者收集的血清作为抗体的来源进行，并且使用犬脑肿瘤细胞作为靶细胞。
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