| Arabidopsis Seed Germination Assay with Gibberellic Acid
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Author:
Date:
2016-11-20
[Abstract] This assay analyzes Arabidopsis seed germination in response to gibberellic acid (GA). During seed imbibition, visible physiological changes allow precise determination of germination rate. This protocol utilizes a stereoscopic microscope to improve characterization of seed germination process.
[摘要] 该测定分析响应于赤霉酸（GA）的拟南芥种子萌发。在种子吸水期间，可见的生理变化允许精确测定发芽率。该协议利用立体显微镜来改善种子发芽过程的表征。
[背景] 种子萌发是由植物激素（如GA和脱落酸（ABA））和环境因素控制的植物生命周期的关键过程。种子萌发包括两个生理过程，包括种皮（睾丸）和胚乳破裂。通常，胚乳通过胚根的渗透表明萌发是完全的。以前的研究通常使用胚乳破裂来计算发芽率。然而，种皮破裂也测量种子发芽的进展。该协议利用立体显微镜提供种子发芽的可见和精确计算，包括种皮和胚乳破裂的速率（Zhong等人，2015）。
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| Cotton Ovules Culture and Analysis
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Author:
Date:
2013-11-20
[Abstract] The cotton ovules culture was innovated by Beasley and Ting (1973), and named after them. It is a convenient system to analyze the effect of chemical or environmental treatment on fiber development directly on ovules. This protocol was generated according to previous published papers and our practical experience.
[摘要] 棉花胚珠文化由Beasley和Ting（1973）创新，并以它们命名。 它是一种方便的系统，用于分析化学或环境处理对直接对胚珠的纤维发育的影响。 该协议是根据以前发表的论文和我们的实践经验生成的。
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