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Author:
Date:
2013-09-05
[Abstract] Chemoresistance is one of the special properties of cancer stem cells, which is the main cause of chemotherapy failure and plays an important role in the recurrence of various cancers including osteosarcoma. The most widely used assay for evaluating chemoresistance is the modified cell proliferation assay. An equal number of cells are seeded onto 96-well culture plates or 24-well plates, and then different concentrations of anti-cancer drugs are added into each well. After that, measure the cell density or cell activity to observe the effect of anti-cancer drugs to cancer cells or cancer stem cells. The chemoresistance of cells is stronger, the cell density or cell activity is higher. Here, we describe chemosensitivity assays for osteosarcoma cells and osteosarcoma stem-like cells.
[摘要] 化学抗性是癌症干细胞的特殊性质之一，其是化疗失败的主要原因，并且在各种癌症（包括骨肉瘤）的复发中起重要作用。 最广泛使用的用于评价化学抗性的测定法是修饰的细胞增殖测定法。 将等量的细胞接种到96孔培养板或24孔板上，然后将不同浓度的抗癌药物加入每个孔中。 之后，测量细胞密度或细胞活性，观察抗癌药物对癌细胞或癌症干细胞的作用。 细胞的化学抗性更强，细胞密度或细胞活性更高。 在这里，我们描述了骨肉瘤细胞和骨肉瘤干样细胞的化学敏感性测定。
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