DNA, SHEARED & DENATURED

DNA，SHEARED和DENATED

Company: AMRESCO

Catalog#: E213

Flotation Assay With Fluorescence Readout to Study Membrane Association of the Enteroviral Peripheral Membrane Protein 2C

Kasturika Shankar, Yuyang Lin and Lars-Anders Carlson

Published online: 2025-04-05

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EMSA Analysis of DNA Binding By Rgg Proteins

Author:

Breah LaSarre and Michael J. Federle,

Date:

2013-08-05

[Abstract] In bacteria, interaction of various proteins with DNA is essential for the regulation of specific target gene expression. Electrophoretic mobility shift assay (EMSA) is an in vitro approach allowing for the visualization of these protein-DNA interactions. Rgg proteins comprise a family of transcriptional regulators widespread among the Firmicutes. Some of these proteins function independently to regulate target gene expression, while others have now been demonstrated to function as effectors of cell-to-cell communication, having regulatory activitiesthat that are modulated via direct interaction with small signaling peptides. EMSA analysis can be used to assess DNA binding of either type of Rgg protein. EMSA analysis of Rgg protein activity has facilitated in vitro ... [摘要] 在细菌中，各种蛋白质与DNA的相互作用对于调节特异性靶基因表达是必需的。电泳迁移率变动分析（EMSA）是允许这些蛋白质-DNA相互作用的可视化的体外方法。 Rgg蛋白包括在坚牢菌中广泛的转录调节子家族。这些蛋白质中的一些独立地起作用以调节靶基因表达，而其它蛋白质已经被证明作为细胞与细胞间通信的效应物起作用，具有通过与小信号肽的直接相互作用调节的调节活性。 EMSA分析可用于评估任一类型的Rgg蛋白的DNA结合。 EMSG对Rgg蛋白活性的分析促进了体外证实调节靶标，通过DNA探针诱变鉴定精确的DNA结合位点，以及表征一些同源信号肽调节Rgg蛋白功能的机制（<例如在某些情况下中断DNA结合）。

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DNA, SHEARED & DENATURED

DNA，SHEARED和DENATED

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