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Author:
Date:
2013-07-20
[Abstract] Polysome profiling is a method that allows monitoring of translation activity of mRNAs in cells and tissues. Once each polysome fractions are collected, the translation activity of each mRNA is analyzed using various molecular biology techniques such as Northern blotting, RT-PCR, microarray or deep-sequencing.
[摘要] 多聚体概况分析是允许监测mRNA在细胞和组织中的翻译活性的方法。 一旦收集每个多核糖体级分，使用各种分子生物学技术如Northern印迹，RT-PCR，微阵列或深度测序分析每个mRNA的翻译活性。
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