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Glycerol 85%

甘油85%

Company: EMD Millipore
Catalog#: 1040941000
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Quantification of Retro- and Lentiviral Reverse Transcriptase Activity by Real-time PCR
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2013-06-05
[Abstract]  Quantification of retroviral reverse transcriptase activity in retrovirus containing supernatant by quantitative reverse transcription PCR as a method for titration of HIV, lenti- and retroviral vectors is described here.. The procedure was optimized for use with LightCycler 480 (Roche, Vilvoorde, Belgium) and ABI 7300 real-time PCR system (reagents and procedures that are system specific will be marked accordingly in the protocol).
[摘要]  本文描述了通过定量逆转录PCR作为滴定HIV,慢病毒和逆转录病毒载体的逆转录病毒逆转录病毒逆转录酶活性的逆转录病毒逆转录酶活性的定量。将该程序优化用于LightCycler 480(Roche,Vilvoorde,Belgium) ABI 7300实时PCR系统(系统特异性的试剂和程序将在协议中相应地标记)

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