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Hausser Scientific Bright-LineTM and Hy-LiteTM Counting Chambers

Hausser Scientific Bright-Line TM和Hy-Lite TM

Company: Thermo Fisher Scientific
Catalog#: 02-671-10
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Clonogenic Assay
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2012-05-20
[Abstract]  Clonogenic assays serve as a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumor cells. A colony is defined as a cluster of at least 50 cells that can often only be determined microscopically. A clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing radiation, but can also be used to determine the effectiveness of other cytotoxic agents. The following protocol has been modified from a published version (Franken et al., 2006). [摘要]  克隆形成测定用作测试给定癌症治疗是否可以降低肿瘤细胞的克隆生存的有用工具。 集落被定义为至少50个细胞的簇,其通常只能在显微镜下确定。 克隆形成测定是选择用于确定用电离辐射处理后的细胞生殖死亡的方法,但也可以用于确定其它细胞毒性剂的有效性。 以下协议已从已发布的版本修改(Franken等人,2006)。

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