{{'Search' | translate}}
 

AquaPhenol

水性酚

Company: MP Biomedicals
Catalog#: AQUAPH01
Bio-protocol()
Company-protocol()
Other protocol()

Synthesis of 5’ end-labeled RNA
Author:
Date:
2012-07-20
[Abstract]  5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not work on 5’ PPP RNA and require a 5’ P substrate. The activity of certain endoribonucleases like Escherichia coli (E. coli) RNase E or Bacillus subtilis (B. subtilis) RNase Y can be stimulated by a 5’ P moiety.
[摘要]  5'末端标记的RNA分子是用于分析各种核糖核酸酶的内切和外切核酸活性的有用底物。 这里给出两个方案来合成具有5'PP或5'P部分的P sup 32标记的RNA。 5'外核糖核酸酶通常不在5'PPP RNA上起作用并且需要5'P底物。 某些内切核糖核酸酶如大肠杆菌(大肠杆菌)核糖核酸酶E或枯草芽孢杆菌(枯草芽孢杆菌 )核糖核酸酶Y可以被5'P部分刺激

Comments