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Crile-Wood Needle Holders

丁腈针架

Company: Fine Science Tools
Catalog#: 12003-15
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In utero Electroporation of Mouse Embryo Brains
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2012-07-20
[Abstract]  This is a non-invasive technique to introduce transgenes into developing brains. In this technique, DNA is injected into the lateral ventricle of the embryonic brains, and is incorporated into the cells through electroporation. Embryos then continue their development in normal conditions in vivo. The effects of genes of interest can be evaluated at certain time points after in utero electroporation. This technique allows acute knockdown or over expression of genes of interest. Compensatory effects from other genes are less likely to happen; it also circumvents possible chronic detrimental effects. [摘要]  这是将转基因引入发育中的大脑的非侵入性技术。 在该技术中,将DNA注入胚胎脑的侧脑室,并通过电穿孔掺入细胞中。 胚胎然后在正常条件下在体内继续其发育。 可以在子宫内电穿孔后的某些时间点评估感兴趣的基因的效应。 这种技术允许急性敲减或过表达感兴趣的基因。 其他基因的补偿效应不太可能发生; 它也绕过可能的慢性有害影响。

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