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Author:
Date:
2012-03-20
[Abstract] Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional FACS scanning allows the differential quantitation of G2 and mitotic cells. Several antibodies to different mitotic markers have been used in the community, including antibodies to MPM-2 antigens present in mitotic cells. MPM-2 recognizes a phosphorylated epitope (LTPLK or YWFSPL) 6, 7 in a distinct class of phosphoproteins including MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The commercial availability and specificity of antibodies to histone H3 phosphorylated at threonine 11, which ...
[摘要] 荧光活化细胞分选（FACS）是一种计数有丝分裂细胞的敏感方法。 细胞用识别仅存在于有丝分裂细胞中的抗原的抗体染色，与碘化丙啶（PI）组合染色DNA。 二维FACS扫描允许差异定量G2和有丝分裂细胞。 已经在社区中使用了针对不同有丝分裂标记物的几种抗体，包括针对有丝分裂细胞中存在的MPM-2抗原的抗体。 MPM-2识别不同类别的磷酸化蛋白中的磷酸化表位（LTPLK或YWFSPL）6,7，所述磷酸化蛋白包括MAP2，HSP70，cdc25和DNA拓扑异构酶IIα，其中大多数在有丝分裂发生时被磷酸化。 在仅存在于有丝分裂细胞中的苏氨酸11磷酸化的组蛋白H3的抗体的商业可用性和特异性也已广泛用于检测有丝分裂细胞。
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