|
Author:
Date:
2012-05-20
[Abstract] Liver is the major site for glycogen storage. Glycogen content can be significantly altered upon disruption of glucose homeostasis in metabolic syndromes, such as diabetes. Glycogen content can be determined by an acid-hydrolysis method (Passonneau and Lauderdale, 1974). Basically, glucose, the hydrolysis product of glycogen, is converted into glucose-6-phosphate (G-6-P) by hexokinase in the presence of ATP. With the supply of NADP, G-6-P is further converted into 6-phosphogluconic acid by G-6-P dehydrogenase (G-6-PDH), while production of NADPH can be measured spectrophotometrically. Our lab has used this method to demonstrate that liver glycogen levels are significantly elevated in diabetic Perk knockout mice (Zhang et al., 2002).
[摘要] 肝是糖原储存的主要部位。 糖原含量可以在代谢综合征例如糖尿病中的葡萄糖体内平衡破坏时显着改变。 糖原含量可以通过酸水解方法测定（Passonneau和Lauderdale，1974）。 基本上，糖蛋白的水解产物葡萄糖在ATP的存在下通过己糖激酶转化为葡萄糖-6-磷酸（G-6-P）。 通过供应NADP，G-6-P通过G-6-P脱氢酶（G-6-PDH）进一步转化为6-磷酸葡萄糖酸，同时可以通过分光光度法测量NADPH的产生。 我们的实验室已经使用这种方法证明在糖尿病Perk敲除小鼠中肝糖原水平显着升高（Zhang等人，2002）。
|