|
Author:
Date:
2016-07-20
[Abstract] Photoreceptors are specialized retinal neurons able to respond to light in order to generate visual information. Among photoreceptors, cones are involved in colors discrimination and high-resolution central vision and are selectively depleted in macular degenerations and cone dystrophies. A possible therapeutic solution for these disorders is to replace degenerating cells with functional cones. Here, we describe a simple protocol for the rapid production of large amount of cone photoreceptors from human pluripotent stem cells. The differentiation protocol is based on the “default pathway” of neural induction using the BMP, TGFβ and WNT antagonist COCO.
[摘要] 光感受器是能够响应光以产生视觉信息的专门的视网膜神经元。 在光感受器中，视锥细胞参与颜色辨别和高分辨率中心视觉，并且选择性缺失黄斑变性和视锥营养不良。 这些疾病的可能的治疗溶液是用功能性锥体代替变性细胞。 在这里，我们描述了一个简单的协议，从人类多能干细胞快速生产大量的锥形感光细胞。 分化方案基于使用BMP，TGFβ和WNT拮抗剂COCO的神经诱导的"默认途径"。
|