| Extraction and Profiling of Plant Polar Glycerol Lipids
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Author:
Date:
2016-06-20
[Abstract] This protocol describes a method to extract total polar glycerol lipids from plant materials, followed by mass spectrometry profiling. Different glycerol lipid classes can be distinguished by their head-groups, which can be profiled automatically and quantitatively by a triple quadrupole mass spectrometry in multiple reaction monitoring (MRM) mode with an autosampler. Comparing with other established methods, such as thin layer chromatography (TLC) separation followed by Gas spectrometry (GC) analysis, this method requires little effort in sample preparation and separation, while the resolution is not limited to general lipid classes but at side chain level. This method was described and used successfully to profile plant lipids changes under freezing stress in Welti et al. ...
[摘要] 该方案描述了从植物材料提取总极性甘油脂质的方法，随后是质谱分析。 不同的甘油脂类可以通过其头基来区分，其可以通过使用自动进样器的多重反应监测（MRM）模式中的三重四极杆质谱自动和定量地进行概况分析。 与其他已建立的方法，例如薄层色谱（TLC）分离，然后气体光谱（GC）分析相比，该方法在样品制备和分离中几乎不需要付出努力，而分辨率不限于一般脂质类别，而是侧链水平 。 在Welti等人（2002）中描述并且成功地使用该方法来分析在冰冻胁迫下植物脂质的变化。
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| Positional Analysis of Fatty Acids in Phospholipids by PLA2 Treatment
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Author:
Date:
2016-06-20
[Abstract] Plant phospholipids can be produced in the endoplasmic reticulum or plastids. Lipids from different sources can be distinguished by the fatty acid profile, in terms of the preferred fatty acid species esterified to the sn-1 or sn-2 position of the glycerol backbone (Ohlrogge and Browse, 1995). This protocol is used to determine the fatty acid profile in total plant phospholipids by the treatment of sn-2 specific phospholipase A2 (PLA2).
[摘要] 植物磷脂可以在内质网或质体中产生。 来自不同来源的脂质可以通过脂肪酸谱来区分，根据酯化为甘油主链的sn-1或em-sn-2位置的优选脂肪酸种类 （Ohlrogge和Browse，1995）。 该方案用于通过处理sn-2特异性磷脂酶A 2（PLA 2）来确定总植物磷脂中的脂肪酸谱。 。
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