29 G 1 mL BD^TM blister, U100 (Australia)

29 G 1mL BD 泡罩，U100（澳大利亚）

Company: BD

Catalog#: 326719

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Reconstitution of Lymphopaenic Mice with Regulatory and Conventional T cell Subsets

Author:

Holly A. Bolton and Barbara Fazekas de St Groth,

Date:

2016-05-20

[Abstract] Transfer of mature T cells into immunodeficient mice results in sub-optimal reconstitution of the peripheral T cell pool. Under lymphopenic conditions, dendritic cells are released from tonic control by regulatory T cells (Tregs), and consequently drive activation and proliferation of low affinity T cells specific for endogenous antigens. This oligoclonal proliferation results in a T cell population dominated by T cells possessing an effector/memory phenotype and a limited TCR repertoire. Oligoclonal expansion can be prevented by selectively reconstituting the Treg compartment prior to T cell transfer (Bolton et al., 2015). Reconstitution of the Treg compartment of lymphopenic mice has been tested in immunodeficient mouse strains such as Rag-1^-/-or Rag-2-/- ... [摘要] 将成熟T细胞转移到免疫缺陷小鼠中导致外周T细胞库的次优重建。在淋巴细胞减少症状下，树突状细胞通过调节性T细胞（Tregs）从强直对照释放，并因此驱动对内源性抗原特异的低亲和力T细胞的激活和增殖。这种寡克隆增殖导致由具有效应/记忆表型和有限TCR库的T细胞支配的T细胞群。可以通过在T细胞转移之前选择性重建Treg区室来防止寡克隆扩增（Bolton等人，2015）。已经在免疫缺陷小鼠品系例如Rag-1 /或 Rag-2 ^{-/- 中测试了淋巴细胞减少小鼠的Treg区室的重建。} 小鼠，以及在通过致死性全身照射作为调节骨髓移植（BMT）的瞬时淋巴细胞减少的免疫小鼠中。将纯化的Treg转移到这些宿主中，结合用外源IL-2处理7天，足以重建Treg区室并减少树突细胞共刺激分子的表达，这是防止自身反应性T细胞不适当扩增的关键过程。在Treg重建后转移的T细胞不经历快速的自发增殖，而是进行慢的内稳态分裂以用天然T细胞重新增殖T细胞库，从而允许外周T细胞库的最佳重建。

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29 G 1 mL BDTM blister, U100 (Australia)

29 G 1mL BD 泡罩，U100（澳大利亚）

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29 G 1 mL BD^TM blister, U100 (Australia)