| RETRACTED: Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection
|
|
Author:
Date:
2020-08-05
[Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health as well as animal health protection. In that aspect, the present protocol focuses on the development of functionalized gold nanoparticle-based lateral flow biosensor for fish nervous necrosis virus (Nodavirus) nucleic acids detection. Total viral RNA, isolated from fish samples was subjected to reverse transcription PCR amplification and the amplification products were mixed with specific oligonucleotide probe. A red test line was formed when ...
[摘要] [摘要] 纸纳米生物传感器已经成为分析导致各种疾病的兽医和人类病原体的一个极好的平台。尤其是侧向流分析或生物传感器是实验室设备和现场分析中灵敏、快速、可靠和准确分析的理想选择。病毒RNA检测对公共卫生和动物健康保护具有重要意义。在这一方面，本协议的重点是开发功能化金纳米粒子侧流生物传感器，用于鱼类神经坏死病毒（Nodavirus）核酸检测。从鱼类标本中分离出的总病毒RNA进行逆转录PCR扩增，扩增产物与特异性寡核苷酸探针混合。当有nodavirus产物存在时，形成红色检测线。这种方法对基础研究有很大的意义，因为它消除了耗时、繁琐的电泳程序的需要，并且可以在养鱼场对养鱼户进行调整。利用这种生物分析平台进行病害监测，无需耗费大量时间和成本，对水产养殖和环境安全有很大影响。
[背景] 关注点和/或现场生物分析一直是关注人类和动物福祉的研究工作的最终目标。基于纸基的传感平台具有功能化简单、重现性好、制造成本低等优点，是一种极具吸引力的分析平台。纸基分析设备已应用于小分子、蛋白质和各种核酸的分析（Parolo和Merkoçi，2013；Bahadir和Sezgintürk，2016；Jiang等人，2019）。侧流生物传感器（LFB）是一种带有干试剂的预制材料条带，通过流体样品激活。它们专为一次性一次性使用而设计，只要有足够的开/关信号（Posthuma ...
|
|
|
| Quantifying Auxin Metabolites in Young Root Tissue of Medicago truncatula by Liquid Chromatography Electrospray-ionisation Quadrupole Time-of-flight (LC-ESI-QTOF) Tandem Mass Spectrometry
|
|
Author:
Date:
2016-06-20
[Abstract] Auxins represent a major group of phytohormones controlling plant development. The spatio-temporal regulation of auxin gradients is essential for the initiation, growth and correct development of plant organs. Because auxins and their metabolites occur at trace levels in plant tissue, experiments requiring identification plus their selective and specific quantification can be most conveniently achieved using mass spectrometry (MS) and the associated chromatographic methods. With the advent of appropriate liquid-based ionisation techniques, emphasis has moved from the use of gas chromatography as the sample interface to the MS (GC/MS), with its concomitant need for derivatisation, to the more sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS). We describe an optimized ...
[摘要] 生长素运输能力的测量提供了涉及植物中生长素运输的生理机制的定量数据。 这种技术很容易执行，并给出快速的结果。 将放射性标记的生长素（吲哚-3-乙酸）通过琼脂块加入到Medic藜苜蓿的根部。 使用液体闪烁计数器测量根中放射性标记的生长素摄取的最终放射性。 在这里，我们描述了生长素运输能力周围的结瘤敏感区在年轻幼苗根的测量。 truncatula 响应根瘤菌接种。 类似的测定可以适用于其他植物物种并回答其他生物学问题。...
|
|
|