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Author:
Date:
2016-04-20
[Abstract] The corn smut pathogen, Ustilago maydis (U. maydis) (DC.) Corda, is a semi-obligate plant pathogenic fungus in the phylum Basidiomycota (Alexopoulos et al., 1996). The fungus can be easily cultured in its haploid yeast phase on common laboratory media. However, to complete its sexual cycle U. maydis strictly requires its specific plant host, maize (Zea mays). The fungus is an interesting and important model organism for the study of the interactions of fungal biotrophic pathogens with plants. In this protocol, we describe the process of plant inoculation, teliospore recovery, germination, progeny isolation and initial mating type analysis. The primary purpose of this protocol is to identify individual progeny strains of U. maydis that can be ...
[摘要] 科斯达（Corda）是在担子菌门（Basidiomycota）中的半必需的植物致病真菌（Alexopoulos等人）中的玉米黑斑病病原体（ al。，1996）。真菌可以容易地在其单倍体酵母相中在普通实验室培养基上培养。然而，为了完成其性周期，U. maydis严格要求其特定的植物宿主，玉米（ Zea mays ）。真菌是研究真菌生物营养性病原体与植物相互作用的有趣和重要的模式生物体。在这个协议中，我们描述植物接种，冬孢子恢复，萌发，后代分离和初始交配型分析的过程。本协议的主要目的是鉴定个体的子代菌株。 maydis ，可用于下游遗传分析。生成目标突变体研究各种过程是这种和许多植物致病真菌的常见方法。通过有性杂交促进产生突变组合的能力，而不需要额外的选择性标记。
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