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Standard System Capillaries, 400 and 600 bar (1100/1200/1260 Series)

标准系统毛细管,400和600 bar(1100/1200/1260系列)

Company: Agilent Technologies
Catalog#: 1100
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Extraction and Quantification of Tryptophan and Kynurenine from Cultured Cells and Media Using a High Performance Liquid Chromatography (HPLC) System Equipped with an Ultra-sensitive Diode Array Detector
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2016-04-05
[Abstract]  Evidence of the involvement of tryptophan and its metabolite, kynurenine, in various biological processes including cancer is constantly expanding. Analysis of cell extracts and culture media can allow for quick snapshots of the metabolic fluctuations occurring in vitro. Here, we describe a method for metabolite extraction from mammalian cells and analysis of extracted metabolites and cell culture media by HPLC with detection using an ultra-sensitive diode array detector. [摘要]  色氨酸及其代谢物,犬尿氨酸参与包括癌症在内的各种生物过程的证据不断扩大。 细胞提取物和培养基的分析可以允许在体外发生的代谢波动的快速快照。 在这里,我们描述了一种从哺乳动物细胞代谢物提取的方法,提取代谢物和细胞培养基通过HPLC检测使用超敏二极管阵列检测器分析。

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