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Author:
Date:
2016-03-20
[Abstract] Fluorescent matrix degradation assay is a popular and widely used assay in the field of invadopodium biology (Artym et al., 2009). Matrix remodeling and degradation can be observed under both physiological and pathological conditions. Cancer cells extensively remodel and degrade the underlying matrix by employing actin-rich protrusive structures called invadosomes. Similar structures are formed by the protozoan parasite Entamoeba histolytica (E. histolytica), upon coming in contact with fibronectin, a major component of the host (extracellular matrix) ECM. Here, we describe a similar assay to measure matrix degradation by Entamoeba histolytica.
[摘要] 荧光基质降解测定是在invadopodium生物学领域中广泛使用的测定（Artym等人，2009）。 可以在生理和病理条件下观察基质重塑和降解。 癌细胞通过采用称为invadosomes的富含肌动蛋白的突出结构广泛地重塑和降解基础矩阵。 在与纤维连接蛋白（宿主（细胞外基质）ECM的主要组分）接触时，由原生动物寄生虫（即溶组织内阿米巴）（ histolytica ）形成类似的结构。 在这里，我们描述了通过溶组织内阿米巴测量基质降解的类似测定法。
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