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Author:
Date:
2016-03-05
[Abstract] Dendritic cells (DCs) play a critical role in mounting the T cell response against different infectious agents. Nature and intensity of the induced T cell responses are defined by activation status of DCs. It is generally accepted that IL-12, IL-4/IL-5 and IL-23 producing DCs induce TH1, TH2 and TH17 type of immune responses, respectively (Kumar et al., 2015). Besides cytokines, levels of co-stimulatory molecules on DCs also influence the response of T cells.
The activation status of DCs can be determined by examining DC culture supernatants for different cytokines and by analyzing expression of co-stimulatory molecules on these cells. However, these approaches provide indirect information about T cell activating potential of DCs. ...
[摘要] 树突细胞（DC）在安装针对不同感染剂的T细胞应答中起关键作用。诱导的T细胞应答的性质和强度由DC的活化状态定义。通常接受的是，产生IL-12，IL-4/IL-5和IL-23的DC诱导T H 1，T H 2和T H （Kumar等人，2015年）中的免疫应答类型。除了细胞因子，DC上的共刺激分子的水平也影响T细胞的响应。通过检查不同细胞因子的DC培养上清液并通过分析这些细胞因子上的共刺激分子的表达，可以确定DC的活化状态细胞。然而，这些方法提供关于DC的T细胞活化潜能的间接信息。在共培养系统中分析T细胞应答是更直接的方法来检查DC的T细胞增殖和极化功效。
在同种异体混合白细胞反应中分析DC的T细胞增殖和极化潜能的方案（allo-MLR）。
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Author:
Date:
2016-02-05
[Abstract] In response to exposure to antigen, T cells whose T cell receptor (TCR) are capable of recognizing the self MHC-antigen derived peptide complex, respond to the antigen and differentiate into one of several subsets, namely TH1, TH2, TH17, Treg, etc. characterized by the signature cytokine they secrete, namely IFN-γ, IL-4, IL-17 or IL-10, respectively, referred to as syngeneic polarization as the MHC presenting the foreign antigen/epitope is self-derived.
T cell responses following incubation for defined periods, usually 3 days for mouse splenocytes, are routinely measured by assessing the antigen-stimulated proliferation of T cells by measuring the radiolabeled precursor thymidine incorporated into the genomic DNA of the dividing T cell; the direction of polarization ...
[摘要] 响应于暴露于抗原，其T细胞受体（TCR）能够识别自身MHC抗原衍生的肽复合物的T细胞应答抗原，并分化为几个亚类之一，即TH1，TH2，TH17，Treg， 等。其特征在于它们分泌的特征性细胞因子，即IFN-γ，IL-4，IL-17或IL-10，分别称为同基因极化，因为呈递外源抗原/表位的MHC是自身衍生的。通常通过测量掺入分裂T细胞的基因组DNA中的放射性标记的前体胸腺嘧啶，通过评估抗原刺激的T细胞增殖来常规测量对于确定的时间段（通常为小鼠脾细胞3天）孵育后的T细胞应答;通过使用培养上清液的ELISA或通过细胞内细胞因子染色随后通过流式细胞术测量由增殖或非增殖应答性T细胞产生的细胞因子来评估极化方向。在下面详述的方案中，我们描述使用的同源小鼠骨髓源性树突状细胞（BMDC）作为APC刺激脾源性T细胞。通过将放射性标记的前体胸苷掺入基因组DNA中来测量T细胞的增殖反应，并通过在72小时期间测量它们分泌的细胞因子即IFN-γ，IL-4和IL-17来评估其极化方向使用ELISA。此外，我们在细胞内细胞因子染色后使用流式细胞术检测CD3 + /CD4 + /CD25低人群中的IL-17阳性T细胞。使用牛分枝杆菌（Mycobacterium bovis） - Bacille Calmette ...
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