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Author:
Date:
2016-02-20
[Abstract] Transmembrane proteins are rarely exclusively localized to a specific vesicle or an organelle. Most transmembrane proteins undergo complicated trafficking routes. Thus, transmembrane proteins are under constant flux, and at steady state, found on a variety of vesicles or organelles. This characteristic makes the study of their trafficking routes complex, since at any given moment, different molecules are often being trafficked in opposing directions. Pulse-chase experiments can temporally track a specific pool of a transmembrane protein of interest, allowing for the kinetic description of its trafficking route. This type of technique has been used extensively to follow a large array of plasma membrane localized proteins (Diril et al., 2006; Jean et al., 2010). Here, we ...
[摘要] 跨膜蛋白很少专门定位于特定的囊泡或细胞器。大多数跨膜蛋白经历复杂的运输路线。因此，跨膜蛋白在恒定通量下，并在稳定状态，发现在各种囊泡或细胞器。这个特征使得他们的贩运路线的研究复杂，因为在任何给定时刻，不同的分子通常在相反的方向上被贩运。脉冲追踪实验可以暂时跟踪感兴趣的跨膜蛋白的特定池，允许其运输路线的动力学描述。这种类型的技术已广泛用于跟踪大量的质膜定位蛋白质（Diril等人，2006; Jean等人，2010）。在这里，我们描述了一种方法，允许研究VAMP8贩运从质膜到内溶酶体隔室。该方法用于描述 MTMR13 和 RAB21 在调节VAMP8向内溶酶体的运输中的作用（Jean等人，，2015）。
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