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Author:
Date:
2016-02-20
[Abstract] The rodent parasite Nippostrongylus brasiliensis (N. brasiliensis) models the salient features of helminth infection including skin penetration, migration from tissues to lung, maturation and egg production in the gut. As a potent activator of systemic and mucosal Th2 immune responses, Nippostrongylus brasiliensis has been extensively used to study host protective immunity and in vivo regulation of Th2 immune response. Six to eight week old C57Bl/6J, Balb/c mice or any other strains are suitable, as all are susceptible to infection. Inocula of 150-650 L3 larvae can be administered by subcutaneous injection, but for greatest consistency a dose of 550 L3 larvae is routinely used for experimental purposes. We have optimized three different protocols for ...
[摘要] 啮齿动物寄生虫（Nippostrongylus brasiliensis）（<巴西> ）模拟蠕虫感染的显着特征，包括皮肤渗透，从组织到肺的迁移，肠道中的成熟和产蛋。作为全身性和粘膜Th2免疫应答的有效激活剂，巴西尼罗巴戟天已广泛用于研究宿主保护性免疫和体内调节Th2免疫应答。 6至8周龄的C57Bl/6J，Balb/c小鼠或任何其它菌株是合适的，因为所有这些菌株都易于感染。 150-650LL幼虫的接种可以通过皮下注射给药，但是为了最大的一致性，通常将550LL幼虫的剂量用于实验目的。我们已经优化了三种不同的方案用于从感染了巴西尼罗巴戟甲L3阶段的小鼠的肺部分离幼虫。幼虫可以从体内任何部位接种后18-60小时迁移到肺部。在接种后48小时，出现在肺峰中的幼虫的数目，并且建议在48小时进行分离/收获以获得每种收获方法的最大一致性：
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