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Author:
Date:
2015-12-05
[Abstract] Legumes are able to form endosymbiotic interactions with nitrogen-fixing rhizobia. Endosymbiosis takes shape in formation of a symbiotic organ, the root nodule. Medicago truncatula (M. truncatula) nodules contain several zones representing subsequent stages of development. The apical part of the nodule consists of the meristem and the infection zone. At this site, bacteria are released into the host cell from infection threads. Upon release, bacteria are surrounded by a host cell-derived membrane to form symbiosomes. After release, rhizobia grow, divide, and gradually colonize the entire host cell of the fixation zone of root nodules. Therefore, mature infected cells contain thousands of symbiosomes, which remain as individual units among other organelles. Visualization ...
[摘要] 豆类能够与固氮根瘤菌形成内共生相互作用。内共生形成共生器官，根瘤的形成。 Medic虫truncatula （ truncatula ）结节包含代表后续发展阶段的几个区域。结节的顶部由分生组织和感染区组成。在这个位置，细菌从感染线程释放到宿主细胞中。释放后，细菌被宿主细胞衍生的膜包围以形成共生体。释放后，根瘤菌生长，分裂，并逐渐定植于根瘤根固定区的整个宿主细胞。因此，成熟的感染细胞含有数千个共生体，其在其他细胞器中保持为单独的单元。可视化的共生体以及受感染的结节细胞中的其他细胞器的组织和动力学是必要的理解机制调节植物和根瘤菌之间的内共生的发展。为了检查这种高度动态的发育过程，我们设计了基于共聚焦扫描显微镜结合不同荧光染料和GFP标记蛋白的有用的成像技术（Gavrin等人，2014）。在这里，我们描述协议的显微镜观察，3D渲染和体积/面积测量的共生体和其他细胞器在感染细胞的M。 truncatula 根瘤。此协议可以应用于监测不同宿主微生物相互作用的发展，无论是共生的还是致病的。
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