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hemocytometer

亮线/暗线计数室

Company: Hausser Scientific
Catalog#: 1483
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In vitro and in vivo Limiting Dilution Assay for Colorectal Cancer
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Date:
2015-11-20
[Abstract]  The in vitro limiting dilution assay is used to determine the colorectal cancer initiating cell (CC-IC) frequency of a CC-IC enriched suspension culture, grown in growth factor enriched serum free media. The in vivo limiting dilution assay is used to determine the colorectal cancer initiating cell frequency of a primary colorectal cancer sample or an established suspension cell line using immunocompromised murine xenograft models. In vitro and in vivo limiting dilution assays (LDAs) can be used to determine the effect of a specific treatment or genetic knockdown strategy on the initiating cell frequency of a population of CC-ICs or colorectal cancer sample, respectively. [摘要]  体外极限稀释测定法用于测定在富含生长因子的无血清培养基中生长的CC-IC富集悬浮培养物的结肠直肠癌起始细胞(CC-IC)频率。 体内限制性稀释测定法用于使用免疫受损的鼠异种移植物模型确定原发性结肠直肠癌样品或建立的悬浮细胞系的结肠直肠癌起始细胞频率。 可以使用体外和体内有限稀释测定法(LDAs)来确定特定处理或遗传击倒策略对CC- IC或结肠直肠癌样品。

A Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts
Author:
Date:
2011-05-20
[Abstract]  This method can be used to free and separate the mesophyll cells from Arabidopsis leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays. [摘要]  该方法可用于从拟南芥叶中游离和分离叶肉细胞。 以这种方式产生的原生质体可用于蛋白质活性和亚细胞定位测定的瞬时表达。

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