| Imaging Thick Lymph Node Tissue Sections
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Author:
Date:
2016-09-20
[Abstract] Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled antibodies. The thick tissue sections (150-200 μm depth) allow for the detection of cell clustering that is typically under-represented in thin sections (10-20 μm) used for conventional confocal microscopy. Application of 2-photon microscopy facilitates imaging through the thick volume of the vibratome sections. In combination with automated image processing software, a thick lymph node cross-section image also facilitates quantitation of cellular events within a relatively large area of the tissue, thus providing a clearer picture on the ...
[摘要] 我们的协议描述了一个简单的程序，通过双光子显微镜成像厚淋巴结切片。 使用vibratome（振动切片机）切割淋巴结以产生组织切片，然后可用荧光标记的抗体染色。 厚组织切片（150-200μm深度）允许检测细胞聚集，其通常在用于常规共聚焦显微镜的薄切片（10-20μm）中表现不足。 双光子显微镜的应用有利于成像通过厚的体积的vibratome部分。 与自动图像处理软件组合，厚的淋巴结横截面图像还有助于在组织的相对大的区域内的细胞事件的定量，从而提供关于感兴趣的细胞事件的空间分布的更清晰的图像（例如 。，T细胞聚类）。 该方法也可以容易地应用于其它组织，例如脾或皮肤。
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| Skeletal Myogenesis in vitro
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Author:
Date:
2015-11-05
[Abstract] Mature skeletal myofibers are elongated and multinucleated cells. Many stem/progenitor cell types, including committed muscle stem (satellite cells) and progenitor (myoblasts) cells, muscle-derived stem cells, myogenic endothelial cells, and mesenchymal stem/stromal cells, have been shown to exhibit skeletal myogenesis under appropriate inductive conditions. Committed muscle stem/progenitor cells and multipotent stem/progenitor cells which have skeletal myogenic capacity can typically be differentiated into skeletal myofibers in vitro following extended low-serum exposure. Differentiated cells exhibit distinct fiber-like elongated morphology with multiple nuclei and express unique muscle molecular markers indicating myogenesis, including desmin (early) and fast- and/or ...
[摘要] 成熟骨骼肌纤维是细长和多核细胞。 许多干/祖细胞类型，包括定型肌干（卫星细胞）和祖细胞（成肌细胞）细胞，肌肉衍生的干细胞，肌肉内皮细胞和间充质干/基质细胞，已显示在适当的诱导条件下表现出骨骼肌发生 。 具有骨骼肌生成能力的承诺的肌肉干/祖细胞和多能干/祖细胞可以在延长的低血清暴露后通常在体外分化成骨骼肌纤维。 分化的细胞表现出不同的纤维样细长形态与多个核，并表示独特的肌肉分子标记指示肌发生，包括结蛋白（早）和快和/或慢肌球蛋白重链（成熟）。
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| Retrograde and Anterograde Tracing of Neural Projections
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Author:
Date:
2013-08-20
[Abstract] Neurons consist of four elements, the soma, dendrite, axon and terminal. They work in concert as the input (soma and dendrite) and output (axon and terminal) parts of neuronal transmission. To function and maintain neuronal activity and metabolisms, proteins and organelles should be transported from soma to terminal via anterograde axonal transport, and also from terminal to soma via retrograde transport. By utilizing these transport systems, neural projection is traced by injecting tracers into local sites of interest. Furthermore, neurochemical properties, such as glutamatergic and GABAergic, can be determined by combining retrograde and anterograde tracing with fluorescent in situ hybridization and immunofluorescence.
[摘要] 神经元由四个元素组成，即索马，树突，轴突和末端。 它们作为神经元传播的输入（神经和树突）和输出（轴突和末端）部分协同工作。 为了起到和维持神经元活动和代谢的作用，蛋白质和细胞器应通过逆行轴突运输从脊髓运输到终末，也可以通过逆行运输从末端转移到血管。 通过利用这些运输系统，通过将示踪剂注入到感兴趣的地方来跟踪神经投影。 此外，神经化学性质，如谷氨酸能和GABA能，可以通过逆行和顺行跟踪与荧光原位杂交和免疫荧光结合来确定。
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