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Author:
Date:
2015-10-20
[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of endothelial cells which are modified by repeated passages and are fully proliferative, a situation far away from physiology. In addition, not only endothelial cells are involved in this process but surrounding cells (such as pericytes, smooth muscle cells, fibroblasts) and the supporting matrix are also major players.
The three-dimensional ex vivo aortic ring model recapitulates the complexities of angiogenesis and combines the ...
[摘要] 血管生成是一种多因素事件，需要内皮细胞的迁移，增殖，分化和结构重排。这种血管生成过程已经使用体外试验例如Boyden腔室试验，伤口愈合试验和管形成试验进行了普遍研究。这些测定主要使用通过重复传代修饰的内皮细胞的单层，并且是完全增殖的，远离生理学的情况。此外，不仅内皮细胞参与这个过程，而且周围细胞（例如周细胞，平滑肌细胞，成纤维细胞）和支持基质也是主要的玩家。
三维主动脉环模型概括了血管生成的复杂性并结合了体外和体内模型的优点。主动脉环在化学上确定的培养环境中培养。在该系统中生长的微血管是具有周围支持细胞的管腔血管，并且与在体内血管生成期间形成的微血管基本上不可区分。可以在不存在血清分子的情况下测定促血管生成因子或抗血管生成因子的功效，否则血清分子可能干扰待测试的物质（Nicosia和Ottinetti，1990）。然而，该系统需要获得新鲜的大鼠组织，但是可以从一个主动脉制备几个样品。
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