| Mitochondrial RNA Transcript Analysis Assay of Arabidopsis Leaf Tissues
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Author:
Date:
2015-10-20
[Abstract] This qPCR-based assay provides an overview of the expression levels of all mitochondrial transcripts (mRNAs and rRNAs) as well as splicing efficiency in Arabidopsis. It was developed before RNAseq techniques were widely used (de Longevialle et al., 2007), but is nevertheless still useful as it is cheaper to run and the analysis is much easier and faster to perform if the aim is only to look at mitochondrial transcripts. For intron-containing mRNAs, the use of primer sets specifically amplifying spliced or unspliced forms allows the evaluation of the splicing efficiency.
[摘要] 这种基于qPCR的测定提供了所有线粒体转录物（mRNA和rRNA）的表达水平以及拟南芥中的剪接效率的概述。 它是在RNAseq技术被广泛使用之前开发的（de Longevialle等人，2007），但是仍然有用，因为它运行更便宜，并且分析更容易和更快地执行，如果目标 只是看看线粒体转录物。 对于含内含子的mRNA，使用特异性扩增剪接或未剪接形式的引物组允许评价剪接效率。
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| Quantification of Retro- and Lentiviral Reverse Transcriptase Activity by Real-time PCR
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Author:
Date:
2013-06-05
[Abstract] Quantification of retroviral reverse transcriptase activity in retrovirus containing supernatant by quantitative reverse transcription PCR as a method for titration of HIV, lenti- and retroviral vectors is described here.. The procedure was optimized for use with LightCycler 480 (Roche, Vilvoorde, Belgium) and ABI 7300 real-time PCR system (reagents and procedures that are system specific will be marked accordingly in the protocol).
[摘要] 本文描述了通过定量逆转录PCR作为滴定HIV，慢病毒和逆转录病毒载体的逆转录病毒逆转录病毒逆转录酶活性的逆转录病毒逆转录酶活性的定量。将该程序优化用于LightCycler 480（Roche，Vilvoorde，Belgium） ABI 7300实时PCR系统（系统特异性的试剂和程序将在协议中相应地标记）
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