| ER Microsome Preparation and Subsequent IAA Quantification in Maize Coleoptile and Primary Root Tissue
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Author:
Date:
2016-05-05
[Abstract] Auxin is a major growth hormone in plants and the first plant hormone to be discovered and studied (Darwin and Darwin, 1880). The auxin molecule in plants was first identified as indole-3-acetic acid (IAA) by Kögl et al. (1934). Active research over nearly a decade has shed light on many of the molecular mechanisms of its action but the complexity and redundancy of the auxin biosynthetic network raises questions about control of this system. We have shown that some enzymes involved in the YUCCA-route of auxin biosynthesis are not cytosolic but localised to the endoplasmic reticulum (ER) in both Arabidopsis thaliana (YUCCA4.2) (Kriechbaumer et al., 2012) as well as Zea mays (ZmTAR1 and ZmSPI) (Kriechbaumer et al., 2015). This ...
[摘要] 生长素是植物中的主要生长激素和待发现和研究的第一种植物激素（Darwin和Darwin，1880）。植物中的植物生长素分子首先由Kögl等人确定为吲哚-3-乙酸（IAA）。 （1934）。近十年的积极研究揭示了其作用的许多分子机制，但是生长素生物合成网络的复杂性和冗余性提出了对该系统的控制的问题。我们已经显示，参与植物生长素生物合成的YUCCA途径的一些酶不是细胞质的，而是定位于拟南芥中的内质网（ER）（YUCCA4.2）（Kriechbaumer等人。（ Zm TAR1和 Zm SPI）（Kriechbaumer /em>，2015）。这提高了生长素生物合成的亚细胞区室的有趣的可能性。为了显示玉米生长素生物合成确实可以在来自玉米幼苗的微粒体以及胞质细胞部分中发生，我们应用本文所述的方案：从玉米胚芽鞘和原代根组织分离微粒体，使用色氨酸的微粒体和胞浆部分的酶测定（Trp）或吲哚-3-丙酮酸（IPyA）作为底物，并提取并定量生长素IAA。
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| In vitro Studies: Inhibition of Nevirapine Metabolism by Nortriptyline in Hepatic Microsomes
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Author:
Date:
2015-10-05
[Abstract] One of the most prevalent and interfering psychosocial comorbidities of HIV infection is clinical depression (22 to 45%). For this reason, a study of a possible interaction between the nonnucleoside reverse transcriptase inhibitor nevirapine (NVP) and the tricyclic antidepressant nortriptyline (NT) was carried out. In vitro studies with rat and human hepatic microsomes showed a marked inhibition of NVP metabolism by NT being more intense in rat than in human. The extrapolation of these results to humans suggests increased NVP side effects when both drugs are coadministered, but additional in vivo human studies are required to evaluate the clinical implication of this interaction.
This protocol describes a technique for detecting and measuring the inhibition of ...
[摘要] HIV感染的最普遍和干扰的心理社会共病是临床抑郁症（22％至45％）。 为此，进行了非核苷逆转录酶抑制剂奈韦拉平（NVP）和三环抗抑郁药去甲替林（NT）之间可能的相互作用的研究。 使用大鼠和人肝微粒体的体外研究显示在大鼠中NT比在人中更强烈的NVP代谢的显着抑制。 将这些结果外推到人表明当两种药物共同给药时，NVP副作用增加，但是需要另外的体内人体研究来评价这种相互作用的临床意义。
该方案描述了 检测和测量肝微粒体中去甲替林对奈韦拉平代谢的抑制的技术。
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