| Measurement of Glucose-6-phosphate Dehydrogenase Activity in Bacterial Cell-free Extracts
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Author:
Date:
2016-10-05
[Abstract] Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.49) is the first enzyme of the oxidative pentose phosphate cycle and catalyses the conversion of glucose-6-phosphate (G6P) to 6-phosphoglucono-δ-lactone and transfers one electron to NADP+ producing one NADPH. Conversion of G6P to 6-phosphoglucono-δ-lactone is proportional to the production of NADPH. The increase in NADPH concentration results in an increase in absorbance at 340 nm. To assay G6PDH activity, therefore, production of NADPH is determined by measuring increase in absorbance at 340 nm spectrophotometrically. This increase rate is then converted to unit of activity and specific activity of G6PDH. In this procedure, a generalized method is given for bacterial G6PDH assays emphasizing on a cyanobacterium Synechocystis ...
[摘要] 葡萄糖-6-磷酸脱氢酶（G6PDH）（EC 1.1.1.49）是氧化戊糖磷酸循环的第一个酶，并催化葡萄糖-6-磷酸（G6P）转化为6-磷酸葡萄糖酸-δ-内酯并将一个电子 到NADP +产生一种NADPH。 G6P向6-磷酸葡萄糖酸-δ-内酯的转化与NADPH的产生成比例。 NADPH浓度的增加导致340nm处吸光度的增加。 因此，为了测定G6PDH活性，通过分光光度法测量340nm处吸光度的增加来确定NADPH的产生。 然后将该增加速率转化为G6PDH的活性和比活性的单位。 在该程序中，给出了强调蓝细菌集胞藻的细菌G6PDH测定的一般方法。 PCC6803（Schaeffer和Stanier，1978; Karakaya等人，2008，2012）和异养细菌大肠杆菌（Hylemon和Phibbs，1972; Barnel等人 ，1990）。
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| In vitro Studies: Inhibition of Nevirapine Metabolism by Nortriptyline in Hepatic Microsomes
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Author:
Date:
2015-10-05
[Abstract] One of the most prevalent and interfering psychosocial comorbidities of HIV infection is clinical depression (22 to 45%). For this reason, a study of a possible interaction between the nonnucleoside reverse transcriptase inhibitor nevirapine (NVP) and the tricyclic antidepressant nortriptyline (NT) was carried out. In vitro studies with rat and human hepatic microsomes showed a marked inhibition of NVP metabolism by NT being more intense in rat than in human. The extrapolation of these results to humans suggests increased NVP side effects when both drugs are coadministered, but additional in vivo human studies are required to evaluate the clinical implication of this interaction.
This protocol describes a technique for detecting and measuring the inhibition of ...
[摘要] HIV感染的最普遍和干扰的心理社会共病是临床抑郁症（22％至45％）。 为此，进行了非核苷逆转录酶抑制剂奈韦拉平（NVP）和三环抗抑郁药去甲替林（NT）之间可能的相互作用的研究。 使用大鼠和人肝微粒体的体外研究显示在大鼠中NT比在人中更强烈的NVP代谢的显着抑制。 将这些结果外推到人表明当两种药物共同给药时，NVP副作用增加，但是需要另外的体内人体研究来评价这种相互作用的临床意义。
该方案描述了 检测和测量肝微粒体中去甲替林对奈韦拉平代谢的抑制的技术。
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