L-Tryptophan, [phenyl-¹⁴C(U)]-

L-色氨酸，[苯基-14C（U）] -

Company: Moravek

Catalog#: MC 2335

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[14C]-Tryptophan Metabolic Tracing in Liver Cancer Cells

Author:

Krishna S. Tummala and Nabil Djouder,

Date:

2015-09-05

[Abstract] Nicotinamide adenine dinucleotide (NAD⁺) is a coenzyme for many NAD⁺-consuming proteins with diverse biological functions. Oscillations in NAD⁺ levels may influence several cellular signaling pathways. NAD⁺ synthesis via Preiss-Handler route (salvage reactions) has been extensively reported. However, the contribution of L-tryptophan/kynurenine catabolism in de novo NAD⁺ synthesis is poorly understood. Using L-[14C]-tryptophan tracing in four liver cancer cell lines and siRNA-mediated silencing of arylformamidase (AFMID), a key enzyme involved in L-tryptophan degradation, we demonstrate the contribution of L-tryptophan catabolism in de novo synthesis of NAD⁺ pools. NAD⁺ modulation is therefore important in ... [摘要] 烟酰胺腺嘌呤二核苷酸（NAD ⁺）是许多具有不同生物功能的NAD ^{+消耗蛋白的辅酶。 NAD ⁺水平的振荡可能影响几种细胞信号传导途径。通过Preiss-Handler途径（补救反应）的NAD +合成已被广泛报道。然而，对L-色氨酸/犬尿氨酸分解代谢在从头NAD +合成中的贡献知之甚少。在四种肝癌细胞系中使用L- [14 C] - 色氨酸示踪和siRNA介导的芳基甲酰胺酶（AFMID）的沉默，其是参与L-色氨酸降解的关键酶，我们证明L-色氨酸分解代谢在从头合成中的贡献 NAD ⁺池。因此，NAD ⁺调节在维持细胞稳态和根据营养物可用性的适当的细胞功能中是重要的。}

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L-Tryptophan, [phenyl-14C(U)]-

L-色氨酸，[苯基-14C（U）] -

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L-Tryptophan, [phenyl-¹⁴C(U)]-