| Macrophage Polarization by Tumor-induced MDSCs Assay
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Author:
Date:
2016-08-20
[Abstract] Myeloid derived suppressor cells (MDSCs) are a subset of granulocytes (immature myeloid cells) that exploit a variety of mechanism to modulate the innate and adaptive immune system. MDSCs are present normally in the body, but their numbers increase during inflammation and in cancer, promoting an immunosuppressive microenvironment. In addition to MDSCs, macrophages also play an important role during cancer development. There are two subsets of tumor associated macrophages (TAMs): M1 and M2. M1 are “anti-tumor” macrophages that are activated by interferon gamma (IFN-γ) and/or Lipopolysaccharide (LPS) and secrete high amount of interleukin 12 (IL-12) thereby inducing a Th1 anti-tumor immune response. M2 or “pro-tumorigenic” macrophages are activated by interleukin 4 (IL-4) and interleukin 10 ...
[摘要] 骨髓衍生的抑制细胞（MDSC）是粒细胞（未成熟骨髓细胞）的子集，其利用多种机制调节先天和适应性免疫系统。 MDSC通常存在于体内，但它们的数量在炎症和癌症期间增加，从而促进免疫抑制微环境。除了MDSC，巨噬细胞也在癌症发展中发挥重要作用。肿瘤相关巨噬细胞（TAM）有两个亚类：M1和M2。 M1是由干扰素γ（IFN-γ）和/或脂多糖（LPS）活化并分泌大量白细胞介素12（IL-12）从而诱导Th1抗肿瘤免疫应答的"抗肿瘤"巨噬细胞。 M2或"致肿瘤发生"巨噬细胞被白介素4（IL-4）和白细胞介素10（IL-10）激活并分泌大量的IL-10，这促进肿瘤进展（Gabrilovich等人， 。，2012）。在肿瘤微环境中MDSC和巨噬细胞之间的相互作用显示增强这些亚群介导的免疫抑制。 MDSC通过产生IL-10而影响TAM，其继而诱导IL-12的下调并将M1极化为M2巨噬细胞。在我们的研究中，我们使用以下方案来评价肿瘤诱导的MDSC将LPS活化的M1极化为M2巨噬细胞的能力（Vences-Catalan等人，2015）。该方案改编自先前的研究（Sinha等人，2007）。
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| Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model
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Author:
Date:
2015-08-20
[Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when (subcutaneously) s.c. grafted into syngeneic C57BL/6 host mice (Foster et al., 1997). This protocol will describe the adoptive transfer of purified CD11b+Gr1+ double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3+ T cells in the TRAMP-C2 prostate cancer mouse model in order to establish the intrinsic functionality of these immune cells and to determine their role in tumorigenesis in vivo ...
[摘要] 免疫细胞用于癌症，慢性感染和自身免疫的过继转移是一个新兴领域，其在最近的试验中显示出希望。 转基因腺癌小鼠前列腺（TRAMP）是前列腺癌（PCa）的经典小鼠模型，TRAMP细胞系衍生自TRAMP小鼠肿瘤。 当皮下注射时，TRAMP-C2是致瘤的。 移植到同基因C57BL/6宿主小鼠中（Foster等人，1997）。 该方案将描述纯化的CD11b +（Ad）双重阳性（DP）骨髓衍生的抑制细胞（MDSC）和CD3 + sup/+的过继转移。 T细胞在TRAMP-C2前列腺癌小鼠模型中的表达，以便建立这些免疫细胞的内在功能并且确定它们在体内的肿瘤发生中的作用（Yan等人）。 ，2014）。
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