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10 ml gastight syringes

10毫升气密注射器

Company: Hamilton
Catalog#: 1010
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Chemotaxis and Jumping Assays in Nematodes
Author:
Date:
2015-09-20
[Abstract]  Nematodes have sensitive olfactory perception, which is used to detect and differentiate many volatile odorants. Some odorants are attractive, others repulsive, and yet others evoke no particular response. Chemotaxis assays can be used to determine the role of certain odors in many different behaviors including foraging, predator avoidance, and mate attraction. In addition to chemotaxis, some species of nematodes in the entomopathogenic genus Steinernema can jump, which is thought to play an important role in host-seeking and dispersal (Dillman and Sternberg, 2012). Jumping and chemotaxis assays have been successfully used to identify odorants that stimulate these behaviors in a variety of nematodes (Bargmann et al., 1993; Campbell and Kaya, 1999; Hallem et al., ... [摘要]  线虫具有敏感的嗅觉感知,其用于检测和区分许多挥发性气味剂。 一些气味有吸引力,其他排斥,还有一些没有引起特别的反应。 趋化性测定可用于确定某些气味在许多不同行为中的作用,包括觅食,捕食者回避和配偶吸引。 除了趋化性之外,昆虫病原属Steinernema中的一些种类的线虫可以跳跃,这被认为在宿主寻找和分散中起重要作用(Dillman和Sternberg,2012)。 跳跃和趋化性测定已经成功地用于鉴定在多种线虫中刺激这些行为的气味剂(Bargmann等人,1993; Campbell和Kaya,1999; Hallem等人, 2011; Dillman等人,2012; Castelletto 等人,2014)。 这里基于文献的成长体提供了趋化性和跳跃测定的详细方案。

Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
Author:
Date:
2015-08-05
[Abstract]  The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E. coli) and subsequent affinity purification using a maltose binding protein (MBP) tag. The proteins can be used in SUMOylation reactions, where the MBP part of the protein can be detected with a commercially available antibody, or additional purification steps can be applied. [摘要]  蛋白质PIAL1(At1g08910)和PIAL2(At5g41580)是最近发现的植物E4 SUMO连接酶的成员。 该方案允许在大肠杆菌(大肠杆菌)中快速和简单地表达重组蛋白,并随后使用麦芽糖结合蛋白(MBP)标签进行亲和纯化。 蛋白质可用于SUMO化反应中,其中蛋白质的MBP部分可用市售的抗体检测,或可应用额外的纯化步骤。

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