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BioMag Goat Anti-Rat IgG

BioMag山羊抗大鼠IgG

Company: QIAGEN
Catalog#: 310107
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Enrichment of Cells of Interest from Heterogeneous Murine Cells with BioMag Goat Anti-rat IgG
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Date:
2012-04-05
[Abstract]  BioMag Goat Anti-Rat IgG is a standard BioMag particle coated with polyclonal goat anti-rat IgG antibodies and is highly suited for use in cell sorting methods where a rat IgG antibody is used as a primary antibody. BioMag Goat Anti-Rat IgG can be used to separate the cells of interest from a heterogeneous cell population using negative selection. BioMag Goat Anti-Rat IgG can also be used as a secondary antibody in enzyme immunoassays and radioassays that utilize a rat IgG primary monoclonal antibody.
The efficiency of enrichment by negative selection depends on antigen availability and the total cell population. The author has intensive experience about enrichment of mouse CD4CD8 double negative (DN) thymocytes from total thymocytes which contains only about 2% DN population. ...
[摘要]  BioMag Goat抗大鼠IgG是用多克隆山羊抗大鼠IgG抗体包被的标准BioMag颗粒,并且非常适用于其中将大鼠IgG抗体用作第一抗体的细胞分选方法。 BioMag山羊抗大鼠IgG可用于使用阴性选择从异质细胞群体中分离感兴趣的细胞。 BioMag山羊抗大鼠IgG也可用作酶免疫测定和利用大鼠IgG第一单克隆抗体的放射性测定中的第二抗体。通过阴性选择的富集效率取决于抗原可用性和总细胞群体。 作者有丰富的丰富的小鼠CD4CD8双阴性(DN)胸腺细胞从总胸腺细胞,只含有约2%的DN人口。 通常在一轮富集后,DN人群占总胸腺细胞的80%以上。 然而,骨髓细胞的消耗效率低得多。

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