| Isolation of Lung Infiltrating Cell in Mice
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Author:
Date:
2015-02-20
[Abstract] Inflammatory lung diseases induce strong leukocyte recruitment into the organ, culminating in pneumonia area formation. Here, we describe the protocol for isolation of lung infiltrating cells. Using this assay, we analyzed the lung cell phenotyping by flow cytometry and spontaneous cytokine production by cultivating lung cells ex vivo (Amaral et al., 2014).
[摘要] 炎性肺疾病诱导强烈的白细胞募集到器官中，最终导致肺炎区域形成。 在这里，我们描述的隔离肺浸润细胞的协议。 使用该测定，我们通过流式细胞术和通过离体培养肺细胞的自发细胞因子产生来分析肺细胞表型（Amaral等人，2014）。
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| Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages
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Author:
Date:
2014-09-20
[Abstract] Macrophages are key cells involved in orchestrating host defense against infections. Here, we describe the protocol for a bacterial killing assay in macrophages that can be adapted to any bacterial pathogen. Using this assay, we analyzed the survival of wild-type and mutant strains of Escherichia coli (E. coli) within RAW 264.7 cells, a widely used macrophage cell line. Bacterial mutants defective in intracellular survival within macrophages can be delineated using this assay.
[摘要] 巨噬细胞是参与编制宿主防御感染的关键细胞。 在这里，我们描述的细菌杀伤测定在巨噬细胞，可以适应任何细菌病原体的协议。 使用该测定，我们分析了广泛使用的巨噬细胞系RAW 264.7细胞内的大肠杆菌（大肠杆菌）的野生型和突变株的存活。 可以使用该测定来描绘巨噬细胞内的细胞内存活缺陷的细菌突变体。
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