| Water Deficit Treatment and Measurement in Apple Trees
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Author:
Date:
2015-02-05
[Abstract] Water is considered perhaps the most limiting factor for plant growth and productivity (Boyer, 1982), and climate change predicts more frequent, more severe and longer drought periods for a significant portion of the world in coming years. Unfortunately, drought resistance is particularly difficult to measure due in part to the complexity of the underlying biology that contributes to a plant’s ability to cope with water limitations. For example, water deficit is frequently examined by detaching leaves or withholding water for a set period of time prior to tissue collection. Such approaches may elucidate the early stages of drought response but are generally not physiologically relevant for maintenance of drought resistance over a longer period. A more realistic approach is to impose a ...
[摘要] 水被认为可能是植物生长和生产力的最大限制因素（Boyer，1982），气候变化预测未来几年世界上相当大一部分地区的更频繁，更严重和更长的干旱期。不幸的是，抗旱性特别难以测量，部分原因是基础生物学的复杂性，有助于植物应对水分限制的能力。例如，在组织收集之前，通常通过将叶子或保留水分离一段设定的时间来检查水缺乏。这些方法可以阐明干旱反应的早期阶段，但是通常在较长时期内维持抗旱性不是生理学相关的。更现实的方法是用持续的土壤水分水平强加逐步的水限制，特别是在多年生木本的情况下。我们在这里描述一个协议，在受控的实验室条件下施加长期缺水，允许分子生物学方法来了解木本植物如何生存的严重水限制。代表性数据可以在Artlip等人（1997）和Bassett等人（2014）中找到。
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| Cotton Ovules Culture and Analysis
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Author:
Date:
2013-11-20
[Abstract] The cotton ovules culture was innovated by Beasley and Ting (1973), and named after them. It is a convenient system to analyze the effect of chemical or environmental treatment on fiber development directly on ovules. This protocol was generated according to previous published papers and our practical experience.
[摘要] 棉花胚珠文化由Beasley和Ting（1973）创新，并以它们命名。 它是一种方便的系统，用于分析化学或环境处理对直接对胚珠的纤维发育的影响。 该协议是根据以前发表的论文和我们的实践经验生成的。
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