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Collagen I 96 Well Clear Flat Bottom TC-Treated Microplate

胶原蛋白I 96孔清晰平底TC治疗微孔板

Company: Corning
Catalog#: 356698
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Hepatitis C virus Cell-to-cell Spread Assay
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2014-12-20
[Abstract]  Hepatitis C virus (HCV) can infect naïve cells via entry of “cell-free” extracellular virus or direct “cell-to-cell” transmission. Here, we describe an assay for detecting HCV cell-to-cell transmission, using a non-growing cell culture system that avoids confounding effects of cell growth. The assay consists of infecting a small number of cells in a confluent monolayer and then blocking subsequent cell-free extracellular virions with a neutralizing antibody such that only cell-to-cell transmission may occur. Under these conditions, incubation at 37 °C results in the formation of infected cell foci. The extent of cell-to-cell spread can then be determined by counting the number of cells in each focus. The assay may be modified to assess the effects of inhibitors and/or specific cellular ... [摘要]  丙型肝炎病毒(HCV)可通过"无细胞"细胞外病毒的进入或直接的"细胞到细胞"传递而感染初始细胞。 在这里,我们描述检测HCV细胞到细胞传播,使用一个非增长的细胞培养系统,避免细胞生长的混杂效应的测定。 该测定法包括感染汇合单层中的少量细胞,然后用中和抗体阻断随后的无细胞的细胞外病毒粒子,使得仅可发生细胞与细胞的传递。 在这些条件下,在37℃下孵育导致感染的细胞病灶的形成。 然后可以通过计数每个焦点中的细胞数量来确定细胞与细胞扩散的程度。 可以修改测定以评估抑制剂和/或特异性细胞基因对HCV的细胞与细胞扩散的影响。

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