| Generation and Selection of Transgenic Olive Plants
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Author:
Date:
2017-11-20
[Abstract] Olive (Olea europaea L.) is one of the most important oil crops in the Mediterranean basin. Biotechnological improvement of this species is hampered by the recalcitrant nature of olive tissue to regenerate in vitro. In previous investigations, our group has developed a reliable Agrobacterium-mediated transformation protocol using olive somatic embryos as explants (Torreblanca et al., 2010). Embryogenic cultures derived from radicles of matured zygotic embryos are infected with Agrobacterium tumefaciens, AGL1 strain, containing a binary plasmid with the gene of interest and the nptII selection gene. After a meticulous selection procedure, carried out using solid and liquid media supplemented with paromomycin, the putative transformed ...
[摘要] 橄榄油（Olea europaea L.）是地中海盆地最重要的石油作物之一。生物技术的改善受到橄榄组织在体外再生的顽固性质的阻碍。在以前的研究中，我们小组开发了一个可靠的土壤杆菌介导的转化方案，使用橄榄体细胞胚作为外植体（Torreblanca et al。，2010）。含有具有感兴趣基因和nptII选择基因的二元质粒的根瘤农杆菌（Agrobacterium tumefaciens）AGL1菌株感染源自成熟合子胚胎胚根的胚性培养物。经过细致的选择程序，使用补充巴龙霉素的固体和液体培养基进行，建立推定的转化系。其转基因性质的初步确认是通过PCR扩增进行的。之后，可以通过有效的再生方案获得植物，其主要特征是使用低离子强度的矿物制剂，在液体培养基中用于培养物同步的阶段以及使用半渗透性乙酸纤维素膜进行胚胎成熟（Cerezo et al。 ，2011）。转基因插入的最终确认通过Southern或Northern分析使用再生植物的叶样品进行。
【背景】由Torreblanca等人开发的协议。 （2010）与Rugini等人（2000）开发的先前的橄榄转化协议有所不同，主要是外植体的种类，土壤杆菌菌株及其选择方法。 Rugini等人（2000）使用胚性质量作为外植体，将其在LBA4404农杆菌菌株的细菌悬浮液中温育48小时。感染后，将外植体在水中漂洗并在补充有250mg ...
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| Agrobacterium-mediated Transformation of Mature Ginseng Embryos
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Author:
Date:
2014-12-20
[Abstract] Ginseng refers to species within the genus Panax and is a slow-growing perennial herb from the Araliaceae family. The most widely used Panax species is Panax ginseng Meyer (Korean ginseng). Panax japonicus (Japanese ginseng), Panax notoginseng (Chinese ginseng), and Panax quinquefolium (American ginseng). Due to the various pharmaceutical importance of ginsenosides, ginseng plant has been cultivated for its highly valued root over 2,000 years as a medicinal plant in East Asian countries particularly in China, Korea, and Japan and North America. Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Cultivar “Yunpoong” has characteristics to have more axillary shoots ...
[摘要] 人参是指属于Panax的物种，并且是来自五加科（Araliaceae）的缓慢生长的多年生草本植物。最广泛使用的人参是人参 Meyer（高丽人参）。 （日本人参），三七（中国人参）和中国人参[美国人参]。 由于人参皂苷的各种药物重要性，人参植物作为一种药用植物在东亚国家，特别是在中国，韩国，日本和北美地区作为药用植物已经培育了其高价值的根。高丽人参（人人参C. A. Meyer ）由来自Jakyung，Chungkyung和Hwangsook三个品系的9个品种组成。栽培品种"云蓬"与其他栽培品种相比具有腋芽和侧根更多的特点。因此"Yunpoong"：人参种子在基因转化过程中对不定根的再生相对更可行。在这里，我们描述如何在收获后准备和处理人参种子，直到人参未成熟胚准备好发芽，并用于根癌土壤杆菌介导的基因转化。
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| Agrobacterium-mediated Transformation of Strawberry
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Author:
Date:
2014-01-05
[Abstract] Traditional breeding for improvement of strawberry (Fragaria x ananassa) is difficult because strawberry is an octoploid, hybrid species. Genetic modification of strawberry would though be a promising alternative for obtaining the desired improvements in existing elite strawberry cultivars (Schaart et al., 2011). The availability of suitable genes for trait improvements in strawberry has however been a rate-limiting step until recently, but with the completion of the sequencing of the genome of woodland strawberry (F. vesca) (Shualev et al., 2011), we now have access to a treasure chest with valuable candidate genes. For strawberry, methods for genetic transformation have originally been described by Nehra et al. (1990) and James et al. ...
[摘要] 由于草莓是八倍体，杂种物种，用于改善草莓（ Fragaria x ananassa ）的传统育种是困难的。草莓的遗传修饰虽然是获得现有的优良草莓栽培品种所期望的改进的有希望的替代方法（Schaart等人，2011）。然而，随着草地草莓基因组测序的完成（Shualev ），用于草莓中性状改善的合适基因的可用性是速率限制步骤 et al。，2011），我们现在可以获得宝贵的候选基因宝库。对于草莓，遗传转化的方法最初由Nehra等人（1990）和James等人（1990）描述，并且转化的成功被证明是高度的品种依赖。草莓转化的最新进展由Husaini等人审查（2011）。在我们的实验室中，草莓的转化是基于由Passey等人（2003）描述的苗再生方法和使用超强毒力土壤杆菌菌株AGL0（Lazo et al。，1991）。我们主要利用草莓转化作为候选基因功能分析的工具。为此，栽培品种Calypso是非常合适的基因型，因为其高转化效率（高达100％）和永不结实的特性，一旦植物开始开花，其提供草莓果实的连续供应。
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