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HYGROMYCINE B VIAL

水杨酸B VIAL

Company: Duchefa Biochemie
Catalog#: H0192
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Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
Author:
Date:
2018-06-05
[Abstract]  Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other microorganisms living in close vicinity of the plant roots. Here we present a protocol on how to prepare DNA suitable for advanced molecular analysis on the soil-borne pathogen Plasmodiophora brassicae, a peculiar unicellular plant pathogenic organism, causing disease on Crucifers. First, it is important to grow Brassica or Arabidopsis plants in infested soils below a temperature of 25 °C under moist conditions to promote root gall formation. Root galls should be harvested ahead of initiation of the decomposing ... [摘要]  从专性营养型土壤植物病原体中分离DNA是具有挑战性的。这是因为他们对植物生长和繁殖的严格要求。土壤栖息地进一步增加了居住在植物根部附近的其他微生物污染的风险。在这里,我们提出了一个关于如何制备适用于土传病原体Plasmodiophora brassicae ,一种特殊的单细胞植物致病生物,导致十字花科病的DNA进行DNA分析的方案。首先,在潮湿条件下,在温度低于25°C的条件下,在感染的土壤中种植芸薹属植物或拟南芥属植物对于促进根gall形成是重要的。在分解过程开始之前,不应迟于接种拟南芥或芜菁植株后四或九周,收获根gall。数量减少的土壤生物的休息孢子通过匀浆gall组织的梯度离心获得。用70%酒精和一套不同的抗生素治疗可促进 P。芸苔纯度。基于CTAB的程序允许分离适合大规模平行测序分析的高质量DNA。

【背景】Plasmodiophora brassicae 是一种土壤传播的植物病原体,其在包括拟南芥属的十字花科家族中引起根虫(棒状杆菌)。根肿病对全世界油菜(油菜)和卷心菜的种植有重大影响。 P上。 brassicae 是指定给超级组Rhizaria的一种专性生物营养素(需要一种生长寄主),Rhizaria是研究最少的真核生物组之一(Sierra等人,2016; Sibbald和Archibald, 2017年)。系统发育上, ...

Agrobacterium-mediated Transformation of Mature Ginseng Embryos
Author:
Date:
2014-12-20
[Abstract]  Ginseng refers to species within the genus Panax and is a slow-growing perennial herb from the Araliaceae family. The most widely used Panax species is Panax ginseng Meyer (Korean ginseng). Panax japonicus (Japanese ginseng), Panax notoginseng (Chinese ginseng), and Panax quinquefolium (American ginseng). Due to the various pharmaceutical importance of ginsenosides, ginseng plant has been cultivated for its highly valued root over 2,000 years as a medicinal plant in East Asian countries particularly in China, Korea, and Japan and North America. Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Cultivar “Yunpoong” has characteristics to have more axillary shoots ... [摘要]  人参是指属于Panax的物种,并且是来自五加科(Araliaceae)的缓慢生长的多年生草本植物。最广泛使用的人参是人参 Meyer(高丽人参)。 (日本人参),三七(中国人参)和中国人参[美国人参]。 由于人参皂苷的各种药物重要性,人参植物作为一种药用植物在东亚国家,特别是在中国,韩国,日本和北美地区作为药用植物已经培育了其高价值的根。高丽人参(人人参C. A. Meyer )由来自Jakyung,Chungkyung和Hwangsook三个品系的9个品种组成。栽培品种"云蓬"与其他栽培品种相比具有腋芽和侧根更多的特点。因此"Yunpoong":人参种子在基因转化过程中对不定根的再生相对更可行。在这里,我们描述如何在收获后准备和处理人参种子,直到人参未成熟胚准备好发芽,并用于根癌土壤杆菌介导的基因转化。

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