{{'Search' | translate}}
 

PEPTONE

PEPTONE

Company: Duchefa Biochemie
Catalog#: P1328
Bio-protocol()
Company-protocol()
Other protocol()

Bacterial Growth Inhibition Assay for Xanthomonas oryzae pv. oryzae or Escherichia coli K12 Grown together with Plant Leaf Extracts
Author:
Date:
2016-12-20
[Abstract]  We performed a growth inhibition assay to test antibacterial compounds in leaf extracts from transgenic rice plants. The assay is based on over-night co-incubation of a defined concentration of colony forming units (cfu) of the respective bacteria together with aqueous extracts of ground leaf tissue. [摘要]  我们进行了生长抑制测定,以测试来自转基因水稻植物的叶提取物中的抗菌化合物。该测定是基于相应细菌的确定浓度的集落形成单位(cfu)与地面叶组织的水提取物的过夜共培养。

背景 对抗有害生物体的植物的防御可以针对特定病原体物种或病原体群体。针对增加作物病原体抗性,对抗特定疾病的育种可能是有用的,但最终目标是实施广谱抗病性。大米蛋白OsJAC1是一种模块化蛋白质,其由预测结合糖残基的茉莉酸相关凝集素结构域和可能在单粘菌偶联期间起作用的活性结构域组成。该融合蛋白特异于禾本科,代表一种新型的抗性蛋白。本文描述的方案用于评估过表达OsJAC1 cDNA cDNA(Weidenbach等人,2016)的转基因水稻植物的叶提取物的抗微生物能力。对于该测定,细菌实验室菌株大肠杆菌K12和细菌水稻病原体xanthomonas oryzae pv。使用oryzae 使用PXO86引起细菌性枯萎病。

Agrobacterium-mediated Transformation of Mature Ginseng Embryos
Author:
Date:
2014-12-20
[Abstract]  Ginseng refers to species within the genus Panax and is a slow-growing perennial herb from the Araliaceae family. The most widely used Panax species is Panax ginseng Meyer (Korean ginseng). Panax japonicus (Japanese ginseng), Panax notoginseng (Chinese ginseng), and Panax quinquefolium (American ginseng). Due to the various pharmaceutical importance of ginsenosides, ginseng plant has been cultivated for its highly valued root over 2,000 years as a medicinal plant in East Asian countries particularly in China, Korea, and Japan and North America. Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Cultivar “Yunpoong” has characteristics to have more axillary shoots ... [摘要]  人参是指属于Panax的物种,并且是来自五加科(Araliaceae)的缓慢生长的多年生草本植物。最广泛使用的人参是人参 Meyer(高丽人参)。 (日本人参),三七(中国人参)和中国人参[美国人参]。 由于人参皂苷的各种药物重要性,人参植物作为一种药用植物在东亚国家,特别是在中国,韩国,日本和北美地区作为药用植物已经培育了其高价值的根。高丽人参(人人参C. A. Meyer )由来自Jakyung,Chungkyung和Hwangsook三个品系的9个品种组成。栽培品种"云蓬"与其他栽培品种相比具有腋芽和侧根更多的特点。因此"Yunpoong":人参种子在基因转化过程中对不定根的再生相对更可行。在这里,我们描述如何在收获后准备和处理人参种子,直到人参未成熟胚准备好发芽,并用于根癌土壤杆菌介导的基因转化。

Comments